乳清酸磷酸核糖转移酶和结直肠癌化疗毒性的相关性

Expressions of orotate phosphoribosyltransferase in colorectal carcinoma and its correlations with toxicities of chemotherapy

目的研究结直肠癌患者乳清酸磷酸核糖转移酶(OPRT)的表达及其同化疗毒性的相关性。方法应用逆转录-聚合酶链式反应(RT-PCR)法检测58例结直肠癌组织及癌旁正常组织OPRT的表达,同时观察患者接受FOLFOX6方案化疗的毒性,分析二者的相关性。结果结直肠癌组织OPRT的表达高于相应正常肠粘膜组织,差异具有显著性(P=0.001)。结直肠癌组织OPRT的表达状况和化疗反应无明显相关性(P>0.05)。正常组织OPRT水平和腹泻的发生呈正相关,并具有统计学意义(P=0.013)。结论结直肠癌组织OPRT的表达和结直肠患者的化疗毒性无相关;而癌旁正常组织OPRT的表达可以预测5-FU相关毒性的发生情况。

Objective To investigate the expression of orotate phosphoribosyltransferase （OPRT） in colorectal carcinoma and analyze its correlations with the toxicities of chemotherapy. Methods The expression of OPRT mRNA was detected using RT-PCR in colorectal carcinoma tissues and paired adjacent normal tissues from 58 patients receiving FOLFOX6 regimen chemotherapy. The toxicities of the chemotherapy were recorded, and the correlations between OPRT mRNA expression and the toxicities were analyzed. Results OPRT mRNA expression was significantly higher in the tumor tissues than in the corresponding normal tissues （P=0.001）, but OPRT expression in the tumor tissues was not correlated with the toxicities of the chemotherapy （P〉0.05）. OPRT level in the normal tissues showed a significant positive correlation with the occurrence of diarrhea in these cases （P=0.013）. Conclusion OPRT expression in colorectal carcinoma tissues is not correlated with the toxicities of 5-FU-based regimen, but OPRT expression in the normal tissues can help predict the toxicities associated with 5-FU.