鼻渊舒口服液对铜绿假单胞菌生物膜体外形成的抑制作用

The effect of biyuanshu oral liquid on the formation of pseudomonas aeruginosa biofilms in vitro

目的:观察鼻渊舒口服液对铜绿假单胞菌生物膜体外形成的抑制作用。方法:平板法建立铜绿假单胞菌细菌生物膜体外模型,银染法及扫描电子显微镜鉴定。不同浓度的鼻渊舒口服液及红霉素作用于成熟前阶段的及已形成的铜绿假单胞菌生物膜,银染法及连续稀释法菌落计数观察其对生物膜的抑制作用。结果:扫描电镜观察铜绿假单胞菌在硅胶片上7d形成生物膜,与银染结果一致。红霉素及鼻渊舒体外能抑制铜绿假单胞菌生物膜的形成,且抑制作用随药物浓度的增加而加强,但对已形成的细菌生物膜清除作用不明显。连续稀释法菌落计数结果表明,不同浓度红霉素及鼻渊舒能抑制成熟前的生物膜膜内细菌生长,与对照组比较差异有统计学意义(P<0.05)。结论:鼻渊舒口服液及红霉素体外对铜绿假单胞菌生物膜的形成有抑制作用。

Objective：To observe the effect of biyuanshu oral liquid on the formation of pseudomonas aerugi- nosa biofilms in vitro. Method： Pseudomonas aeruginosa biofilm was established by plate culture and detected by Scanning electron microscopy and AgNO3 stainning . After treated with different dosages of biyuanshu oral liquid and erythromycin, the pseudomonas aeruginosa biofilms were observed by AgNO3 stainning and the number of via- ble bacteria were measured by serial dilution. Result： The pseudomonas aeruginosa biofilms could be detected by SEM at the seventh culture day and it was consistent with the detetion of AgNO3 stainning. The biyuanshu oral liquid and erythromycin have the effect on inhibiting the formation of pseudomonas aeruginosa biofilms. But with the already formed pseudomonas aeruginosa biofilms the inhibition was not significant. The serial dilution method showed that the viable counts of bacteria of biyuanshu oral liquid and erythromycin treated groups were significant- ly lower than those untreated groups（P〈0.05）. Conclusion：The biyuanshu oral liquid and erythromycin can inhib- it the formation of pseudomonas aeruginosa biofiims in vitro.