降钙素基因相关肽阳性神经纤维在鼠颞下颌关节中的分布

Distribution of calcitonin gene related peptide like immunoreactive fibres in the temporomandibular joint of rat

目的 观察鼠颞下颌关节中降钙素基因相关肽阳性神经纤维的分布特征。方法 将鼠颞下颌关节取出 ,脱钙脱水后恒冷切片 ,以ABC法漂染后进行镜检及半定量分析。结果 除关节盘中心区外 ,颞下颌关节各部位的软组织均有丰富的降钙素基因相关肽阳性纤维 ,主要沿血管尤其是动脉周围分布 ,密度为 :前部盘周附着和关节囊 (45 4 6± 72 8)mm2 、后部 (30 2 7± 43 4)mm2 、外侧 (2 40 2± 2 3 6 )mm2 、内侧 (2 2 9 6± 2 5 0 )mm2 、盘外周区 (2 0 2 4± 35 2 )mm2 。结论 降钙素基因相关肽阳性纤维广泛分布于颞下颌关节中 ,是感觉神经的一部分 。

Objective To observe the distribution of calcitonin gene related peptide like immunoreactive (CGRP LI) nerve fibres in rat's tempormandibular joint (TMJ) Methods The avidin biotin peroxidase complex method and image analysis were employed to detect CGRP LI nerve fibres in frozen sections of TMJs in eight rats Results CGRP LI nerve fibres were plentifully distributed in TMJs except the central disc band and bone, mainly in the periphery of blood vessels, especially arteries Density: anterior disc attachment and capsule, (454 6±72 8) mm 2; posterior portion, (302 7±43 4)mm 2; lateral portion, (240 2±23 6)mm 2; medial portion, (229 6±25 0)mm 2; and peripheral portion of disc, (202 4±35 2)mm 2 Conclusion CGRP LI nerve fibres were widely distributed in rat TMJ and were a part of trigeminal sensory nerve Density was highest in anterior disc attachment and capsule