反义寡核苷酸对血管平滑肌细胞c-myc、增殖细胞核抗原蛋白表达的影响

Effect of protein expression on antisense c-myc and PCNA for vascular smooth muscle cells

目的 研究反义c myc、增殖细胞核抗原 (PCNA)基因片断对血管平滑肌细胞 (VSMCs)增殖的作用。 方法 使正义、反义及错配反义c myc、PCNA ,分别作用于体外培养的VSMCs ,应用蛋白印迹和免疫组化法测定并经计算机图像分析 ,观察其对c myc、PCNA蛋白表达的影响。 结果 10μmol/L浓度的反义c myc、PCNA作用于VSMCs后 1～ 5d ,相应c myc和PCNA蛋白表达减弱 ,计算机图像分析表明其表达产物与对照组相比 ,差异有显著意义 (P <0 0 5 ) ,而正义和错配反义寡核苷酸无此效应 (P >0 0 5 )。 结论 反义c myc、PCNA寡核苷酸可通过阻遏其相应基因表达进而抑制蛋白表达及VSMCs增殖。

Objective To study the methods of inhibiting proliferation of vascular smooth muscle cells(VSMCs), we observed the change of protein expression after using antisense c myc, PCNA for VSMCs. Methods The sense, antisense, and mismatched oligomers for c myc or PCNA were individually delivered to the cultured VSMCs. Using Western blot, immunohistochemistry assay and image analysis system, we observed the change of c myc and PCNA protein expression in the VSMCs. Results Antisense c myc, PCNA at concentration of 10 μmol/L can successfully inhibit the expression of c myc, PCNA protein in the VSMCs ( P <0 05),but the sense and the mismatched do not have this effect ( P >0 05). Conclusion The data suggested that the antisense c myc, PCNA could attenuate the protein expression of c myc, PCNA, and it is an effective method for the inhibition of VSMCs hyperplasia.