烤烟种质资源遗传多样性和群体结构及亚群特异性研究

Study on genetic diversity,population structure and specificity of subpopulations of flue-cured tobacco germplasm

筛选多态性丰富的16对SRAP引物,对不同地理来源的276份烤烟资源的遗传变异进行分析,结果表明:16对SRAP引物共检测出611个等位位点,平均每对引物对38.19个,多态性较高;国外烤烟品种遗传多样性高于国内品种,贵州烤烟品种遗传多样性水平较低;NJ法聚类、PCA分析和STRUCTURE群体结构分析结果相互吻合,证明烤烟是与地理来源和亲缘关系密切相关的资源群,贵州烤烟资源形成了独立的组群,其遗传背景独特;各烤烟群体拥有独立的特有和特缺等位位点,群体间互补等位位点丰富,利用国外品种来拓宽国内烤烟品种的遗传基础最具潜力。

Genetic diversity of 276 flue-cured tobacco germplasm of worldwide origin was evaluated based on 16 selective polymorphic prirrler pairs. Results showed that a total of 611 alleles were amplified by using 16 SRAP primer pairs, with an average of 38. 19 alleles per primer pair. Flue-cured tobacco accessions from abroad showed higher diversity than those from China. Flue-cured tobacco from Guizhou province showed the lowest diversity. Results obtained by NJ cluste-ring, PCA, and STRUCTURE analysis were identical, which indicated that flue-cured tobacco were a closely related group consistent with geographic origins and genetic relationship. Unique and deficient alleles in each population were detected and plenty of complementary alleles among geographic populations were found. Foreign accessions had the most potential to broaden the genetic basis of flue-cured tobacco accessions from China.