聚羟基丁酸酯载体人工软骨体内培育的实验研究

Tissue engineered neocartilage using polymer substrates and chondrocytes:experimental study

目的 探讨聚羟基丁酸酯 (PHB)泡沫材料作为软骨细胞支架材料以及体内培育组织工程化人工软骨的可行性。 方法 取 4周龄雄性新西兰幼兔关节软骨 ,胶原酶消化 ,将所获软骨细胞种植到PHB泡沫材料上 ,体外培养 1周后 ,将细胞 支架材料复合体移植到兔背部皮下 ;以单纯植入聚羟基丁酸酯泡沫材料及接种软骨细胞组为对照组。分别于术后第 4、8、12周取材 ,进行大体观察及组织学、免疫组织化学观察。 结果 软骨细胞 支架材料复合体组在兔背部皮下有新生软骨形成 ,两个对照组无软骨形成。 结论 PHB泡沫材料可以作为软骨组织工程的人工细胞外基质 。

Objective To investigate the posibility of using polyβ hydroxybutyate (PHB) as a scaffold for chondrocytes and the feasibility of generating tissue engineered cartilage in rabbit animal model. MethodsThe articular cartilage from 4 week old rabbits was enzymatically dissolved by type II collagenase,and chondrocytes were harvested and seeded into foams of PHB. After incubation for 1 week in vitro, chondrocyte polymer constructs were implanted subcutaneously into rabbits. Two respective control groups were established by subcutaneous implantation of PHB foams and chondrocytes alone. The rabbits were killed at 4,8,12 weeks postoperatively, and the specimens were disected and examined macroscopically, histologically and immunohistochemically. [Results Specimens harvested from chondrocyte polymer constructs and subjected to gross morphologic and histologic analysis demonstrated new cartilage formatiom,and those from control groups showed no cartilage formation. ConclusionThese findings demonstrate that the foam of PHB is not only a good “matrix” for cartilage tissue engineering, but also optimal scalf fold for the seeding of chondrocytes to generate new cartilage that would be useful in plastic and reconstructive surgery.