海藻酸钠纯度对微囊化肝细胞腹腔移植中微囊纤维化的影响

Effectofalginate purity onmicroencapsulated hepatocyte overgrowth in peritoneal transplantation

目的 研究海藻酸钠纯度对微囊纤维化的影响。 方法 采用逐步过滤和氯仿 /丁醇萃取的方法纯化海藻酸钠 ,用纯化和未纯化的海藻酸钠制作两种微囊化肝细胞分别给两组大鼠 (各2 0只 )腹腔移植 ,观察不同时期微囊的回收率、微囊纤维化、囊内肝细胞的功能和结构。用3 H TdR掺入法测定纯度对淋巴细胞转化率的影响。 结果 纯化组的微囊回收率较未纯化组明显增高 (P <0 0 1)。纯化组移植 1个月后大部分肝细胞形态及酶组化染色正常。未纯化组移植后 1个月内肝细胞破坏。移植 6个月时 ,纯化组微囊仍保持完整光滑 ;而未纯化组移植 1个月后微囊全部纤维化。纯化的海藻酸钠对人类淋巴细胞转化率明显低于未纯化组。

Objection To study the effect of alginate purity on micro encapsule overgrowth in peritoneal transplantation. Methods Alginate was purified by filtration and chloroform/butanol extraction. Using purified and crude alginate to make two kinds of microencapsulated hepatocytes (MHs), we transplanted 5 ml of each kind of MHs into the peritoneal cavities of rats. After 2 weeks,1, 2 and 6 months, MHs were washed out to study overgrowth of MH, histology, enzyme and albumin of hepatocytes. Human lymphocytes transformation was measured by 3H TdR incorporation with two kinds of alginate solutions. ResultsThe amount of retrieved MHs was larger in the purified than in the crude( P <0 01). In the purified groups, most hepatocytes had normal features of morphology, enzymatic histochemistry, and albumin immunohisto chemistry staining after one month of transplantation, but were damaged in the crude groups only 2 weeks later. Microencapsules were smooth, regular and had no overgrowth during 6 months in the purified but overgrowth one month later after transplantation in the crude. The liquid scintillation counting (cpm) was lower in the purified than in the crude. Conclusion The purified alginate could reduce the overgrowth of MH and improve the function of hepatocytes significantly.