胃上皮细胞凋亡与消化性溃疡愈合的关系

Relationship of gastric epithelial cell apoptosis and peptic ulcer healing

目的 观察幽门螺杆菌 (HP)阳性的胃溃疡病人在溃疡愈合前后胃上皮细胞凋亡及HP检测的情况。方法 采用检测DNA裂解的末端脱氧核苷酸转移酶 (TdT)介导的dUTP缺口末端标记法 (TUNEL) ,在原位标记凋亡的胃上皮细胞。结果 溃疡愈合后胃上皮细胞凋亡指数下降 ,与愈合前相比两者间有显著性差异 (P <0 0 5 )。溃疡愈合后HP全部阴转 ,HP的检出密度与胃上皮细胞凋亡指数之间呈正相关性 (r=0 .80 ,P <0 0 5 )。结论 HP在体内能诱导胃上皮细胞凋亡 。

Objective To investigate the gastric epithelial cell apoptosis and Helicobacter pylori (HP) detection in the patient showing the positive HP with peptic ulcer before and after ulcer healing.Methods By use of the method of terminal deoxynucleotidyl (TdT) mediated dUTP nick ending labelling (TdT mediated dUTP nick ending labelling)which is adopted to detect DNA splitting,we labelled apoptosis gastric epithelial cell.Results The gastric epithelial cell apoptosis index decreased after the ulcer healing.There was significant difference between before and after the ulcer healing( P <0.05).The HP detection all turned negative after the ulcer healing.There was positive correlation between the density of HP and the gastric epithelial cell apoptosis index(r=0.80, P <0.05).Conclusion HP can induce gastric epithelial cell apoptosis and result in formation and recurrence of ulcer.