过氧亚硝基阴离子致豚鼠气道反应性增高的实验研究

PEROXYNITRITE ANION INDUCES HYPERREACTIVITY OF GUINEA-PIG TRACHEAS IN VITRO

目的 探讨过氧亚硝基阴离子（ＯＮＯＯ－）在气道上皮细胞损伤致气道高反应性中的作用。方法 以离体豚鼠气管条建立组织胺累积－ 浓度反应曲线，电镜观察气道上皮细胞的形态学变化。结果 豚鼠气管条与ＯＮＯＯ－ 孵育后，气管条的反应性增高，同时电镜观察有气道上皮细胞损伤脱落。结论ＯＮＯＯ－ 参与了气道上皮细胞的损伤，并导致了气道高反应性的形成。

ObjectiveTo investigate the effect of peroxynitrite anion(ONOO -) on airway hyperreactivity.MethodsConcentration-response curves of histamine on contraction of tracheal strips of the guinea-pig were constructed and the morphological changes of tracheal epithelial cells were observed with electron microscopy after incubation with peroxynitrite.ResultsThe exposure of tracheal strips to peroxynitrite led to epithelial damage and hyperreactivity to histamine.ConclusionPeroxynitrite may be involved in the tracheal epithelial damage and airway hyperreactivity.