HMGB1和VEGF-C在宫颈鳞癌中的表达及意义

The Expression and Significance of HMGB1 and VEGF - C Protein in Human Cervical Squamous Cell Carcinoma

目的探讨高迁移率族蛋白B1(HMGB1)、血管内皮生长因子C(VEGF-C)在宫颈鳞癌中的表达及其意义。方法采用免疫组织化学技术检测HMGB1、VEGF-C在76例宫颈鳞癌、15例宫颈上皮内瘤变(CIN)III期及15例正常宫颈鳞状上皮中的表达,统计分析其表达水平与临床病理参数的关系。结果从正常宫颈鳞状上皮、CIN III到宫颈鳞癌组织,HMGB1的阳性表达率逐渐增高(P<0.01)。HMGB1的表达在临床分期晚、有淋巴结转移和肿瘤直径大的宫颈鳞癌组织中明显增高(P<0.05),而与病理分级无关(P>0.05)。从正常宫颈鳞状上皮、CIN III到宫颈鳞癌组织,VEGF-C的阳性表达率逐渐增高(P<0.01)。VEGF-C的表达在临床分期晚、有淋巴结转移、病理分化差和肿瘤直径大的宫颈鳞癌组织中明显增高(P<0.05)。HMGB1与VEGF-C在宫颈鳞癌中的表达呈正相关(P<0.01)。结论 HMGB1与VEGF-C在宫颈鳞癌组织中均呈过表达并密切相关,二者在宫颈鳞癌的发生发展、侵袭及淋巴转移中起重要作用。

Objective To investigate the expression and significance of HMGB1 and VEGF-C in human cervical squamous cell carcinoma（CSCC）.Methods The expression of HMGB1 and VEGF-C were monitored by immunohistochemical staining in 76 cases with CSCC,15 cases with CIN III（cervical intraepithelial neoplasia） and 15 cases with normal cervical squamous epithelial tissue samples.The relationships between their expression and the clinical pathological parameters of CSCC were analyzed.Results From normal cervical squamous epithelial tissue,CIN III to CSCC,the positive rates of HMGB1 showed an increased trend（P 〈 0.01）.The expression of HMGB1 was significantly higher in patients with advanced stage,lymphatic metastasis and bigger size of the tumor（P 〈 0.05） in CSCC.From normal cervical squamous epithelial tissue,CIN III to CSCC,the positive rate of VEGF-C showed an increase trend（P 〈 0.01）.The expression of VEGF-C was significantly higher in patients with advanced stage,lymphatic metastasis,bigger size of the tumor and lower differentiative squamous carcinoma（P 〈 0.05） in CSCC.The expression of HMGB1 was positively correlated with the expression of VEGF-C（P 〈 0.01）.Conclusion HMGB1 and VEGF-C protein were over-expressed in CSCC.Their over-expression might play a critical role in the initiation,progression,invasion and lymphatic metastasis of CSCC.