全氟辛酸对小鼠肝脏相关酶系的影响

Effects of PFOA on Relevant Enzymes Department in Liver of Mice

目的:观察全氟辛酸对小鼠肝脏相关酶系的影响。方法:将小鼠随机分为4组,分别为空白组、1/16LD50、1/8LD50和1/4LD50组,染毒14 d后采用试剂盒法测定肝组织匀浆中琥珀酸脱氢酶(SDH)、血清中乳酸脱氢酶(LDH)和血清中单胺氧化酶(MAO)含量的变化。结果:染毒组肝组织中SDH活性显著低于空白组(P<0.01),随染毒剂量增加呈递减趋势;染毒组血清中LDH和MAO活性显著高于空白组(P<0.01)。随染毒剂量增加呈递增趋势。结论:全氟辛酸通过损伤肝细胞线粒体,破坏能量代谢,进而损伤肝组织,可致肝坏死。

AIM： To investigate the effects of PFOA on relevant enzymes department in liver of mice. Methods： Mice were divided into 4 groups randomly, the control group, 1/16LD50, 1/8LD50, 1/4LD50 After exposure for 14d, succinate dehydrogenase（SDH）, lactate dehydrogenase（LDH）, Monoamine oxidase（MAO） were measured by the method of reagent＇s directions. Results： Activity of SDH in the model group were lower remarkably than those in the control group（P 〈 0.01 ）, and decreased in a dose-dependent way. Activity of LDH and MAO in the model group were higher remarkably than those in the control group （ P 〈 0.01 ） , and increased in a dosedependent way. Conclusion： PFOA induced liver injury by damaging Mitochondria and energy decompensation, finally caused liver necrosis.