基于核糖体小亚基DNA序列的鼠尾藻系统发生分析

Molecular Phylogeny of Sargassum thunbergii O. Kuntze Inferred from Small Subunit Ribosomal DNA(SSU rDNA) Sequences

通过制备野生鼠尾藻基因组DNA,用同源克隆技术获得鼠尾藻的SSU rDNA序列。该序列长度为1 784 bp,A、G、T、C 4种碱基的含量分别为24.66%、27.75%、26.35%和21.24%,序列已提交至GenBank,登录号为JN604979。选用GenBank数据库中已有的褐藻SSU rDNA序列构建系统发生树,结果显示:鼠尾藻与半叶马尾藻,铜藻以及Sargassum macrocarpum聚为一支,并且与半叶马尾藻的亲缘关系最近。

Genomic DNA of Sargassum thunbergii was extracted and the SSU rDNA sequence was amplified via PCR technique. The sequence results showed that it was composed 1784 nucleartides in which the A, G, T and C contents are 24, 66%, 27.75%, 26.35% and 21.24%, respectively. The sequence has been submitted to GenBank database, and the accesssion number is JN604979. Neighbor-joining phylogenetic trees based on SSU sequences are constructed and S. thunbergii was clustered with Sargassum confusum, Sargassum macrocarpum and Sargassum horneri. S. thunbergii exhibited a very close relationship to S. confusum.