滴水珠组培快繁的实验研究

A Study on Rapid Propagation Technology of Cordate Pinellia Tuber

目的:通过滴水珠组织培养及快速繁殖,解决滴水珠的资源匮乏问题。方法:以滴水珠的珠芽和叶片及叶柄为外植体,进行愈伤组织诱导、愈伤组织分化、丛生芽的增殖、生根诱导及组培苗的移栽研究。采用酸性染料比色法测定滴水珠愈伤组织中总生物碱的含量。结果:滴水珠组织培养的最佳外植体为叶片,在MS+6-BA 2.0 mg/L+NAA 0.2 mg/L的培养基上可以较好地诱导出滴水珠的愈伤组织,滴水珠愈伤分化及丛生芽增殖的最适培养基为MS+6-BA 0.5mg/L+NAA 0.25 mg/L,生根诱导最适培养基为1/2MS+NAA 0.25 mg/L。在培养基中添加100g/L的香蕉汁具有促进滴水珠丛芽增殖及生根的作用。滴水珠愈伤组织中的总生物碱含量为0.784 8%。结论:初步建立了完整的滴水珠的组织培养体系,并成功移栽。

Objective：To solve the lack of resoures of Cordate propagation technology. Method： Using the bulbule, leaves and study the technology of callus induction, callus differentiation, transplantation of tissue culture seedlings ; to determine the total using Acid dye colorimetry. Results： Leaves were the best tissue 6 - BA 2.0 mg/L ＋ NAA 0.2 mg/L is a good medium to induce differentiation and Clustered buds proliferation medium is MS ＋ Pinellia Tuber through tissue cuhure and rapid leafstalk of Cordate Pinellia Tuber as explants to Clustered buds proliferation, root induction and alkaloid content in Cordate Pinellia Tuber callus culture explants of Cordate Pinellia Tuber, MS ＋ callus of Cordate Pinellia Tuber. The best callus 6 - BA 0. 5 mg/L ＋ NAA 0.25 mg/L, and the best roots induce medium is 1/2MS ＋ NAA 0.25 mg/L. It can promote the Clustered buds proliferation and root grows when added 100 g/L to the medium. The total alkaloid in Cordate Pinellia Tuber callus is O. 784 8%. Conclusion： a rapid propagation technology of Cordate Pinellia Tuber is establislled in this study, and the transplantation was successful.