摘要
目的:探讨促性腺激素和细胞因子在上皮性卵巢癌发生发展中的作用。方法:应用四甲基偶氮唑蓝(MTT)实验观察卵泡刺激素(FSH)、黄体生成素(LH),人重组白介素-6(hrIL–6)对卵巢癌细胞株增殖的影响。结果:卵泡刺激素、黄体生成素及人重组白介素-6对卵巢癌细胞株人卵巢低分化乳头状腺癌细胞株(OVCAR-3)、人上皮性卵巢癌细胞株(Caov-3)具有促增殖作用并呈剂量依赖性,与对照组相比差异有显著意义。在卵巢癌细胞培养液中加入50μg/ml抗白介素-6(IL-6)中和性抗体,两种卵巢癌细胞的生长均有所减慢。结论:肌体内促性腺激素水平异常与上皮性卵巢肿瘤形成密切相关,激素与细胞因子影响其发展和转归。
Objective： To investigate the role of gonadotropins and cytokines in the development of epithelial ovarian carcinoma.Methods： Two epithelial ovarian carcinoma cell lines（OVCAR-3,Caov-3） were cultured with follicle-stimulating hormone（FSH）,luteinizing hormone（LH） and human recombinant interleukin-6（hrIL-6）,respectively.The degree of proliferation of two cell lines was determined by MTT.Results：The proliferation was increased by gonadotropins or IL-6 for a period of 96 h（P〈0.05 vs control group） and a dose-dependent effect was seen in both OVCAR-3 and Caov-3.Most significantly,for both cell lines,50 μg/ml anti-IL-6 antibody partially inhibited the hormone-stimulated cell proliferation.Conclusion： Elevated serum gonadotropin is one of the factors contributing significantly to the development of epithelial ovarian carcinoma.Gonadotropins and cytokines are sensitive biomarkers for for the prognosis of the ovarian carcinoma.
出处
《天津药学》
2012年第3期5-7,共3页
Tianjin Pharmacy
