柚皮总黄酮半仿生提取工艺研究

Study on Semi-bionic Extraction Process of Total Flavonoids from Pomelo Skin

对柚皮总黄酮半仿生提取工艺进行了研究。以柚皮总黄酮提取量为考察指标,考察了提取温度、提取时间、液料比(提取液体积与柚皮质量之比)对柚皮总黄酮提取量的影响。在单因素试验和正交试验的基础上,获得半仿生法提取柚皮总黄酮的最佳工艺条件:以pH=2.5的盐酸和pH=7.5、pH=8.0的磷酸氢二钠-柠檬酸缓冲溶液作为提取液,液料比为25 mL/g,70℃下提取60 min,重复提取3次,在此条件下柚皮总黄酮的提取量为50.65 mg/g。半仿生提取法适用于柚皮总黄酮的提取,效果较好,提取率高。

The semi-bionic extraction of total flavonoids from pomelo skin was studied. Experiments were made by using orthogonal design with extraction yield of total flavonoids as the index, investigating the infuence of extracting temperature, time and the ratio of liquid to solid. The optimum process based on the single factor and orthogonal test was as the following： the extracting solvent was pH = 2.5 HC1 solution,pH = 7.5 and pH = 8.0 disodium phosphate-ctric acid bufer solution, the rate of solvent to material was 25 mL/g and extract for three times at 70℃ for 60 rain. And the extraction yield of total flavonoids from pomelo skin is up to 50.65 mg/g at the optimum parameters. Semi-bionic extraction method is suitable for extractine total flavonoids from Domelo skin with hieh extraction efficiency.