8-羟基脱氧鸟苷与多囊卵巢综合征的相关性研究

Association of 8-hydroxydeoxyguanosine with polycystic ovarian syndrome

目的通过测定和比较健康妇女和多囊卵巢综合征（polycystic ovarian syndrome,PCOS）患者血浆中8-羟基脱氧鸟苷（8-hydroxydeoxyguanosine,8-OHdG）水平来评估PCOS患者氧化基本损伤情况。方法确诊为PCOS患者32例（新诊断和没有因多囊卵巢综合征得到任何治疗的患者）,31例健康育龄期女性,PCOS组患者在月经周期第3-5天,闭经者于B超未见优势卵泡时,对照组妇女在月经第3-5天时,收集空腹静脉血8mL,EDTA标凝血2.0mL。采用酶联免疫吸附法（ELISA）法定量检测血液标本中8-OHdG水平。结果 PCOS患者血浆中8-OHdG表达水平显著高于PCOS患者血浆中8-OHdG表达水平显著高于对照组妇女血浆中8-OHdG的水平（[351.79±29.56）v（s207.70±59.08）],差异有显著性（P〈0.05）。32例PCOS患者中BMI〈25的患者20例和其余BMI≥25的患者,血浆中8-OHdG水平的差异无显著性（[353.87±23.82）vs（346.91±23.82,P=0.5891]。结论在年龄和体重指数无明显差异的情况下,PCOS患者血浆中8-OHdG的表达高于正常妇女,并可能与BMI无明显关系。

[Objectives] The aim of this study was to investigate the PCOS patients＇oxidative DNA damage by testing and comparing the 8-OhdG levels of patients with PCOS and healthy control subjects. [Methods] 32 diag- nosed PCOS, then 31 normal fertile women were selected as control group. To PCOS patients, samples were collected in the 3-5 days of menstrual cycle, amenorrhea＇s unseen dominant follicle in B ultrasonic, while to control group, 8 mL of blood, 5 mL of urine and 2 mL of EDTA were collected in the 3-5 days of menstrual cycle. 8-OhdG in blood was quantitatively detected by ELISA. [ Results ] The 8-OHdG levels in blood of those patients with PCOS were significantly higher than healthy controls [（351.79±29.56）vs（207.70±59.08）], The difference between these groups was statistically significant （P 〈0.05）. Among those PCOS patients, the difference of the 8-OHdG levels in blood between 20 patients with BMI〈25 and others with BMI≥25 was no statistically significant[（353.87±23.82） vs （346.91±23.82）, P =0.5891]. [Conclusions] Research indicates that the former＇s expression of 8-OhdG was higher than the latter while their age and BMI were no statistically significant, and was no associated with BMI.