正交试验法优化马兰头叶蛋白的提取工艺研究

Study on Optimization of the Extraction Technology of Protein from the Kalimeris indica(L.) Sch. -Bip.by Orthogonal Test

采用碱提酸沉法从马兰头中提取蛋白质。在单因素试验的基础上,采用L9(34)正交试验设计,研究pH、温度、水解时间和料液比对马兰头蛋白提取率的影响。结果表明,在各影响因素中,影响程度依次为:pH>温度>水解时间>料液比,碱法提取马兰头蛋白的最佳工艺条件为:pH 9.0、提取温度50℃、水解时间60min、料液比1︰25(g/mL),在此条件下测得马兰头蛋白提取率为75.86%。

The protein was extracted from Kalimeris indica（L.） Sch.-Bip.by alkali extraction.Based on single factor test,the effects of pH,extraction temperature,extraction time,extraction solid/liquid ratio（g/mL） on the extraction rate of protein were studied according to L9（34） orthogonal test.The results showed that factors influencing the extraction of protein from the Kalimeris indica（L.） Sch.-Bip.by alkali-extracting method were in the order as follows： pHextraction temperatureextraction solid/liquid ratio（g/mL）extraction time,the optimum extracting conditions of protein from the Kalimeris indica（L.） Sch.-Bip.for orthogonal test were adding 25 times amount water solutions of pH 9.0 and extracting for 60 min at 50℃,and the extraction rate of protein was 75.86%.