摘要
以阳春砂仁(Amomun villosum)为原料,经过研磨过筛,超声浸提,分级萃取,得到砂仁粗提物.研究提取物的抑菌性和抗氧化性,结果表明,砂仁提取物对枯草芽孢杆菌(Bacillus subtilis)、大肠杆菌(Escherichia coli)、沙门氏菌(Salmonella)、铜绿假单胞菌(Pseudomonas aeruginosa)、葡萄球菌(Staphylococcus aureus)和肺炎克雷伯菌(Klebsiella pneumoniae)有不同程度的抑制作用.1,1-二苯基苦基苯肼(DPPH)实验显示,砂仁提取物具有较强的抗氧化作用,以乙酸乙酯层提取物的抗氧化效果最好,质量浓度达到18μg/mL时,导致0.1mmol/L DPPH自由基下降到13.99%,其清除率为86.01%.
The antimicrobial and antioxidant effects of extract from Amomum villosurn Lour have been studied using the methods of sift through the grinding, ultrasonic extraction, hierarchical extraction to get villosum extract. The results showed that extract of A. villosum have different degrees of inhibition on Bacillus subtilis, Escherichia coli, Salmonella, Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneurnoniae. DPPH test showed that the extract had strong antioxidant. Extract of A. villo-sum ethyl acetate layer had the best antioxidant effects, and decreased the amount of DPPH （0. 1 mmol/L） free radical to 13.99% when its concentration was 18μg/mL,clearance rate was 86.01%.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第4期789-792,共4页
Journal of Xiamen University：Natural Science
基金
福建省发改委项目(闽发改高技(2009)608号)
福建省科技公益专项(2009R10037-2
2009R10037-3)
福建省科技平台项目(2008Y2003)
关键词
砂仁
提取物
抑菌
抗氧化
Amomum villosum
extract
antibacterial
antioxidant
