解脲脲原体、人型支原体在健康学龄前儿童泌尿生殖道中的分子流行病学研究

Molecular epidemiological investigation of Ureaplasma urealyticum and Mycoplasma hominis in urogenital tract of healthy preschoolers

目的:研究健康学龄前儿童泌尿生殖道解脲脲原体(Uu)及人型支原体(Mh)分子流行病学特征。方法:对408份健康学龄前儿童尿液标本进行Uu、Mh培养,阳性者用聚合酶链反应(PCR)进一步确证,并对扩增阳性的Uu进一步分群与分型。结果:408例标本中,Uu阳性27例(6.62%);Mh阳性3例(0.74%)。Uu单纯生物一群占48.15%(13/27),单纯生物二群占29.63%(8/27),两群混合占22.22%(6/27)。Uu阳性率男性为4.72%(12/254),女性为9.74%(15/154),差异有统计学意义(x^2=3.903,P=0.048)。农村儿童Uu阳性率为10.83%(13/120),城市儿童为4.86%(14/288),差异有统计学意义(x^2=4.889,P=0.027)。结论:Uu与Mh可能属于健康学龄前儿童泌尿生殖道中的正常定植菌群。

Objective： To determine the infection of Ureaplasma urealyticum （Uu） and Mycoplasma hominis （Mh） in urogenital tract of healthy preschoolers. Methods： Urine specimens were collected from 408 preschoolers of kindergartens in Qingdao. All specimens were cultured by Uu and Mh selective liquid media. The positive cases were then amplified by polymerase chain reaction （PCR）. Results： The positivity rate of Uu in healthy preschoolers was 6.62%, and the Mh was 0.74%. Of 27 Uu cases 13 were Parvum biovar （48.15%）, 8 were urealyticum bio- var （29.63%） and 6 mixed biovars （22.22%）. The pesitivity rate of the male was 4.72% （12/254）, while the female was 9.74 % （15/154）, with a marginally significant difference （ P = 0.048）. The isolation rate of Uu among the children from countryside was 10.83% （13/120）, while 4.86% （14/288） were found among the children from city, with a significant difference between the two groups （ P =0.027）. Conclusion： Uu and Mh may be the common symbiotic organisms in lower urogenital tract of healthy preschoolers.