α-氰基丙烯酸酯医用胶在大鼠肝脏短期创面局部反应的研究

Short-term local reaction study of α-cyanoacrylate adhesive on rat liver wound model

目的:研究α-氰基丙烯酸酯医用胶短期肝创面局部反应。方法:试验采用SD雄性大鼠肝表面创伤模型,试验组和对照组分别采用在肝表面创伤部位喷涂医用胶和肝表面创伤部位不做处理方法,于术后5 min、1 d、3 d观察、取材,并进行组织学研究。结果:大体观察:大鼠皮肤切口愈合良好,肝脏手术区大体正常。病理观察:试验组与对照组在术后1 d均有炎症反应,伴有中性粒细胞、淋巴细胞、吞噬细胞渗出,成纤维细胞增生,无坏死发生,但试验组在3 d时炎症较轻,且创伤修复较好。结论:在术后早期,没有发现α-氰基丙烯酸酯医用胶引起局部肝组织坏死,与对照组相比炎症较轻,创伤修复较好。

Objective：To investigate the short - term local reaction of α - cyanoacrylate adhesive on a rat liver wound model. Methods： The SD male rat was applied in this study. Testing medical adhesive was sprayed on the liver trauma surface, while, liver trauma surface of control group did not make any treatments. Rats were sacrificed humanely 5 minutes, 1 day and 3 days post - operation. The local reaction was observed macroscopically and micro- scopically. Results： Gross appearance： There was no abnormal appearance in the operation area. Microscopic ap- pearance： Inflammatory response was observed in both testing and control group after 1 day, accompanied by exuda- tion of neutrophils, lymphocytes and phagocytes, and fibrous cell proliferation ; but necrosis was not found. The med- ical adhesive group displayed minor inflammation and better wound healing than control group after 3 days. Conclu- sion： α -cyanoaerylate adhesive could not cause local liver tissue necrosis. Only minor inflammation was found. The wound healing of testing group was better than that of control group.