藏药短管兔耳草中松果菊苷和麦角甾苷的含量测定

Determination of echinacoside and acteoside in Tibetan herb Lagotis brevituba Maxim

目的:建立RP-HPLC法同时测定松果菊苷和麦角甾苷的含量。方法:采用ZORXDB C18(150 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-0.1%醋酸水溶液(3∶7),流速1.0 mL.min-1,检测波长330 nm,柱温25℃,进样量20μL。结果:本方法可在25 min内完成松果菊苷和麦角甾苷2个成分的色谱分析,且两成分色谱峰之间具有良好的分离度,线性范围分别为25～400μg.mL-1(r=0.9996)和19.4～310μg.mL-1(r=0.9985),平均加样回收率(n=9)分别为99.8%和97.8%。结论:以松果菊苷和麦角甾苷为目标成分时,青海省海北州热水地区为短管兔耳草的最佳采收地。该方法灵敏度高、准确、简便、快速,可用于短管兔耳草的质量控制。

Objective： To develop an RP - HPLC method for the simultaneous determination of echinacoside and acteoside in Tibetan herb Lagotis brevituba Maxim. Methods： The sample was separated on an ZORXDB C18 （150 mm ×4. 6 mm,5 μm） with the mobile phase consisting of methonal -0. 1% acetic acid water solution（3： 7） at the flow rate of 1.0 mL .min^-1 ,UV detection wavelength was set at 330 nm,and the column temperature was 25 ℃. Results：Experimental results showed that a good separation of echinacoside and acteoside was achieved in 25 min by HPLC method ; The method displayed good linearity of echinacoside and acteoside with the correlation coeffi- cients and linear ranges of 25 - 400 μg. mL^-1 （ r = 0. 9996 ） and 19.4 - 310μg .mL ^-1 （ r = 0. 9985 ） ; The aver- age recoveries（ n = 9 ） were 99.8% and 97. 8%. Conclusion： The best harvest region is Reshui of Haibei, when echinacoside and acteoside are taken as target components. The method is sensitive, accurate, simple and fast, and can be used for quality control of Lagotis brevituba Maxim.