HPLC法同时测定不同产地及不同采收时间山茱萸中3个环烯醚萜苷的含量

HPLC simultaneous determination of three iridoid glucosides in Fructus Corni from different sources and different harvest periods

目的:采用高效液相色谱法测定不同产地及不同采收期山茱萸中的莫诺苷、马钱苷及獐牙菜苷3个环烯醚萜苷类的含量。方法:采用Welchrom-C18(250 mm×4.6 mm,5μm)色谱柱,柱温35℃,以甲醇-0.1%磷酸水溶液为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长240 nm。结果:莫诺苷、马钱苷和獐牙菜苷的进样量分别在12.0～600.0,8.64～432.0,3.72～186.0μg.mL-1范围内,与色谱峰面积呈良好线性关系(r=0.9997,n=6);平均回收率(n=6)分别为96.3%,103.4%,98.8%。不同产地的山茱萸中,莫诺苷以西北大学果园采收的含量最高,马钱苷以陕西省丹凤县产的含量最高,獐牙菜苷以西北大学果园采收的含量最高;不同采集时间的西北大学果园山茱萸中莫诺苷、马钱苷和獐牙菜苷的含量范围分别为15.21～50.50,2.72～8.21,0.30～1.43 mg.g-1。结论:本方法操作简单、快速,重复性好,可同时测定山茱萸中莫诺苷、马钱苷和獐牙菜苷的含量。

Objective：To establish an HPLC method for simultaneous determination of three secoiridoid glucosides （morroniside, loganin and sweroside） in Fructus Corni from different sources and during different harvest periods. Methods： Welchrom C18 （250 mm × 4.6 mm, 5 μm） column was used and methanol and water（ containing 1% phosphoric acid） solution as mobile phase in a gradient elution, the flow rate was 1.0 mL · min^-1, the detection wavelength was at 240 nm and the column temperature at 35 ℃. Results： The linear ranges of morroniside, loganin and sweroside were 12.0 -600. 0, 8.64 -432. 0, 3.72 - 186.0 μg . mL^-1μg, respectively（r =0. 9997,n =6）. The average recoveries （n = 6 ） were 96.3 % , 103.4% , 98. 8 % , respectively. The content of morroniside of sample collected from botanical garden of Northwest University is the highest; The content of loganin of sample collected from Shaanxi Danfeng is the highest; The content of sweroside of sample collected from botanical garden of North- west University is the highest. The content ranges of morroniside, loganin and sweroside in Fructus Corni from dif- ferent harvest periods were 15.21 - 50.50, 2.72 - 8.21, 0. 30 - 1.43 mg .g^-1. Conclusions： The validated HPLC method has the advantages of precision, reproducibility and reliability, allowing simultaneous determination and comparison of morroniside, loganin and sweroside in Fructus Corni.