摘要
目的观察鞘内注射钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)抑制剂KN93对瑞芬太尼痛觉过敏大鼠痛行为的影响。方法SD雄性大鼠60只按随机数字表法分为5组:空白对照组(C组,n=12)、切口痛(I组,n=12)、瑞芬组(R组,n=12)、二甲基亚砜组(DMSO组,n=12)以及KN93组(n=12)。除空白对照组外均做切口痛模型,瑞芬组、DMSO组及KN93组切皮同时经皮下泵注瑞芬太尼(0.04mg/kg,1mg溶于40ml生理盐水)30min。DMSO组及KN93组术前30min分别鞘内给予10%DMSO20μl和KN9320μl(50μg溶于20μl10%DMSO中)。各组分别在术前24h及术后2h、6h、24h、48h检测术侧热缩足潜伏期(Pawwithdrawalthermallatency,PWTL)及机械缩足阈值(Pawmechanicalwithdrawalthreshold,PMWT)。结果与C组相比,I组术后各时间点PwTL[(11.24±0.69)s,(10.36±0.29)S,(11.29±1.12)S,(12.21±0.75)S]及PMWT[(25.5±1.20)S,(24.92±1.98)S,(25.47±1.54)s,(27.14±1.04)S]明显降低(P〈0.05);与I组相比,R组术后PWTL[(8.48±0.72)s,(8.58±0.45)S,(8.46±0.92)S,(9.07±0.79)S]及PMWT[(21.2±2.42)S,(19.58±1.12)S,(21.87±1,56)S,(22.26±1.64)s]明显降低(P〈0.05);与R组相比,KN93组术后各点踟汀L[(13.32±0.73)S,(11.79±0.32)S,(11.86±0.98)S,(12.76±0.82)s]及PMWT[(29.75±1.38)S,(28.27±1.16)s,(26.5±1.02)s,(27.79±1.22)s]明显升高(P〈0.05)。结论鞘内注射KN93能够缓解瑞芬太尼诱发的痛觉过敏。
Objective To study the effects of intrathecal injection of CaMK Ⅱ inhibitor KN93 on the hyperalgesia induced by remifentanil in a incision pain model. Methods Sixty SD rats were divided rando'mly into 5 groups ( n = 12) : C group (control) ; I group ( incision pain) ; R group ( incision pain +remifentanil) ; DMSO group ( incision pain + remifentanil + DMSO) and KN93 group ( incision pain + remifentanil). In group R ,. DMSO and KN93 , remifentanil (0.04 mg/kg, 1 mg remifentanil was dissolved in 40 ml NS ) needed to be infused subcutaneously 30 min at the moment of surgery. Group DMSO and group KN93 were respectively intrathecal injected 20μl DMSO( 10% ) and 20μ1 KN93(50 μg/20 μl,dissolved in 10% DMSO). Each rat received tests of the paw mechanical withdrawal threshold (PMWT) and the paw withdrawal thermal latency (PWTL) at the times of 24 h before and 2h,6h,24h,48h after surgery. Results Compared with the group C,the PWTL( ( 11.24 ±0.69) s, (10.36 ±0.29)s,(11.29 ±1.12)s,(12.21 ±0.75)s) and PMWT( (25.5± 1.20)s,(24.92 ± 1.98)s,(25.47 ± 1.54) s, (27.14 ± 1.04) s) of the group I were significantly decreased after surgery(P 〈 0.05 ). Compared with the group I,the PWTL( (8.48±0.72)s,(8.58 ±0.45)s,(8.46 ±0.92)s, (9.07 ±0.79)s and PMWT( (21.2 ± 2.42 ) s, ( 19.58 ± 1.12 ) s, ( 21.87 ± 1.56 ) s, ( 22.26 ± 1.64) s) of the group R were significantly decreased after surgery(P 〈 0.05 ). Compared with the group R, the PWTL ( ( 13.32 ± 0.73 ) s, ( 11. 79± 0.32 ) s, ( 11. 86 ± 0.98)s,(12.76±0.82)s) andPMWT((29.75±1.38)s,(28.27±1.16)s,(26.5±1.02)s,(27.79±1.22) s) of the group KN93 were significantly increased (P 〈 0.05 ). Conclusion Intratheeal injection KN93 ec,uld re- lieve the hyperalgesia induced by remifentanil
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2012年第7期627-629,共3页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金项目(30872439/81171047)
六大人才高峰项目(2010117)
江苏省自然基金项目(BK2010105)
江苏省卫生厅课题项目(RC2011006/H200943)
南京市卫生局重大课题项目(zkx08028)