福州呼吸道感染患儿中人偏肺病毒的检测

Human metapneumovirus infections in children of Fuzhou city,China

目的了解人偏肺病毒(hMPV)在我国福州儿童呼吸道感染中的检出情况。方法收集2007年11月至2010年4月在福建省福州市妇幼保健院因呼吸道感染住院的重症监护病房的137例患儿鼻咽抽取物标本,通过逆转录聚合酶链反应RT-PCR扩增法用一对特异引物对hMPV N基因片段进行检测。结果从137份标本中发现了6份hMPV感染阳性标本。其中2份合并呼吸道合胞病毒(RSV)感染。hMPV感染的检出率为4.4%。hMPV阳性标本中,hMPV与某些呼吸道病毒,如呼吸道合胞病毒(RSV)、流感病毒(甲、乙型)等的混合感染的检出率是33.3%。通过对N基因的213bp检测片段的基因序列分析和比对,表明福州地区检出的这几株hMPV分别属于A、B两种基因型。结论 hMPV是儿童上下呼吸道感染中较为重要的一个病原,在福州地区hMPV的两种基因型同时存在。

Objective To estimate the prevalence of human metapneumovirus（hMPV） infection in children of respiratory tract disease in Fuzhou,Fujian,China.Methods Nasopharyngeal aspirates were collected from children with respiratory tract infections from November 2007 to April 2010.Sequence analyses were done after RT-PCR amplification of a 213-nucleotide fragment of the hMPV N gene to compare with available sequences in GenBank.Results A total of 137 clinical samples were tested for hMPV using RT-PCR.Six were hMPV positive（4.4%） with 2 of them co-infected with respiratory syncytial virus（RSV）.The sequence of the 213-nucleotide fragment of the hMPV N gene from four samples were compared with 4 representative strains in GenBank;it showed that the hMPV strains associated with acute respiratory infection in children of Fuzhou,China can be categorized into genotype A and B.Conclusions The hMPV infection in children with respiratory tract disease is 4.4% and the virus strains are genotypes A and B in Fuzhou,China.