软籽石榴(Punica granatum L. cv. Yushizi)叶片再生体系的研究

Adventitious Shoots Regeneration from Leaves of Soft-seeded Pomegranate(Punica granatum L. cv. Yushizi)

为建立软籽石榴的离体高效再生体系,试验以新梢为材料建立了软籽石榴的无菌体系,并试验了不同激素组合对软籽石榴增殖效果的影响;以叶片为外植体研究了不同培养基组合对不定芽再生频率和伸长的影响。结果表明,茎段增殖培养的最适培养基为MS+6-BA 0.3 mg/L+KT 0.3～0.4 mg/L+NAA 0.1 mg/L;叶片不定芽诱导的最适培养基为MS+TDZ 0.6 mg/L+NAA 0.2 mg/L,在不定芽诱导培养基中添加0.6 mg/L的GA3对不定芽的伸长效果较好。在1/2MS+NAA 0.2 mg/L+IBA 0.2 mg/L+AC0.5 g/L培养基增殖培养所得芽苗生根效果最优。

：To establish the system of rapid in vitro propagation of pomegTanate, stems were used to study the effects of different hormone com- bination on the multiplication of shoots ; the ]eaves were used to study the effects of different hormone combination on the adventitious shoots inducement, then rooting of shoots were studied. The results showed that the optimum media for multiplicatlon of shoots were MS ＋ 6-BA 0. 3 mg/L ＋ KT 0.3-0.4 mg/L ＋ NAA 0. ling/L, the optimum media for adventitious buds inducement were MS ＋ TDZ 0.6 mg/L ＋ NAA 0. 2 rag/L, the adventitious buds grew well on the inducement medium added GA0.6 rag/L, the optimum media for roofing were I/2MS ＋ NAA 0.2 mg/L ＋ IBA 0.2 mg/L ＋ AC 0.5 g/L.