板栗酰基转移酶基因的克隆及其在短雄花序发育中的表达

Cloning and expression of acyltransferase gene from Castanea mollissima during catkin development

板栗短雄花序有利于减少树体营养消耗。雄花短化的机理研究对新种质的培育与利用有重要意义。试验从发育中的板栗芽变短雄花序cDNA中获得一个酰基转移酶cDNA全长。该结构基因包括一个1 335bp的开放阅读框,编码一个含445个氨基酸的完整阅读框架,预测蛋白的相对分子质量约为49kD,无跨膜结构。通过实时荧光定量PCR分析发现该酶在正常花序和芽变花序发育过程中表达量存在差异,短雄花序顶端死亡脱落时该基因表达量显著高于正常雄花序。初步推断在短雄花序生长发育过程中,该酶参与了花序顶端细胞的程序性死亡过程,其较高的表达是造成雄花序顶端死亡的原因之一。

The short catkin of Chinese chestnut （Castanea mollissirna） helps to reduce the nutrition consumption of plant. The research of mechanism on shortened catkin is important to the cultivation and using of new germplasm. In this study, a gene of Acyltransferase from the short-catkin mutant of Castanea mollissima was isolated. The structural gene consisted of a 1 335 bp open reading frame that encoded a protein of 445 amino acids with a calculated molecular weight of 49 kD and had no membrane- spanning region. Expression studies using qRT-PCR showed that the gene expressions during the flower development of Casta- nea mollissirna were different between the mutant and the normal plant, for the expression in the catkins of mutant was higher than the normal one when the top of catkins were dying. It was concluded that the gene might play a role in the programmed cell death in top of the catkins. The higher expression of the gene was one of the reasons for the death of the top of male catkins.