南疆杏品种自交不亲和S-RNase基因型的鉴定

Identification of self-incompatibility S-RNase genotypes for apricot cultivars in South of Xinjiang area

【目的】为鉴定新疆主栽杏品种自交不亲和S-RNase基因型,【方法】以新疆27个主栽杏品种的叶片为试材,利用蔷薇科通用引物组合:EM-PC2consFD+EM-PC3consRD、PaConsⅡ-F+PaConsⅡ-R和PruC2+PruC5对其基因组DNA进行了特异等位PCR扩增,将克隆测序结果在GenBank上进行同源性比对。【结果】结果表明,27个新疆杏品种成功扩增出54个基因片段,其核苷酸序列共包括19个不同的S-RNase基因,其中4个为GenBank上登陆的已知杏属S-RNase基因,分别定名为S24(588 bp)、S14(708 bp)、S23(972 bp)、S21(971bp),15个为新的S-RNase基因,暂时分别命名为S40(572 bp,登陆号:HQ342870、S41(401 bp,登陆号:HQ342871)、S42(357 bp,登陆号:HQ342872)、S43(493bp,登陆号:HQ342873)、S44(657 bp,登陆号:HQ342874)、S45(1 294 bp,登陆号:HQ342875)、S46(830 bp,登陆号:HQ342876)、S47(575 bp,登陆号:HQ342877)、S48(594 bp)(登陆号:HQ342878)、S49(653 bp,登陆号:HQ342879)、S50(659 bp,登陆号:HQ342880)、S51(768 bp,登陆号:HQ342881)、S52(1 520 bp,登陆号:HQ342882)、‘赛买提’S65(594 bp,登陆号:JQ327151)、‘小白杏’S66(709 bp,登陆号:JQ327152)。鉴定的27个新疆杏品种的S-RNase基因型分别为:S23S14(‘晚熟胡安娜’、‘何谢克’、‘贝新纳尔’);S52S14(‘乔尔胖’、‘馒头玉吕克’);S14S47(‘克孜阿恰’、‘元旦杏’、‘辣椒杏’);S23S4(‘莎车黑叶杏’、‘库车托咏’、‘安江胡安娜’);S52S23(‘索格佳娜丽’);S52S51(‘冬杏’);S52S50(‘苏陆克’);S52S40(‘卡巴克胡安娜’);S43S41(‘贾格达玛依桑’、‘洪得克’、‘脆佳娜丽’、‘拉胡安娜’、‘克孜佳娜丽’);S45S42(‘毛拉肖’);S45S46(‘托乎提’);S45S44(‘赛来玉吕克’);S49S24(‘库尔勒托咏’、‘黄肉油杏’);S21S65(‘赛买提’);S52S66(‘小白杏’)。【结论】研究鉴定出27个新疆杏品种自交不亲和雌蕊的S基因型,为杏品种商业化栽培配置适宜授粉树提供了理论依据。

[Objective] In order to identify S-RNase genotypes associated with self-incompatibility of apricot cultivated in Xinjiang, [Method] three pairs of consensus primer EM-PC2consFD＋EM-PC3con- sRD, PaCons 11 -F＋PaCons 11 -R and PruC2＋PruC5 were used to amplify genome DNAs of the leaves from 27 main apricot cultivars, which had been published for S-RNase in Prunus. The specific fragments were cloned and sequenced, and then the nucleotide acid sequences were blasted on GenBank. [Result] The results showed： 54 gene fragments were amplified from 27 apricot cultivars successfully. Searches for ho- mology with our sequences publicly available DNA databases revealed 4 S-RNase gene fragments had been deposited in the GenBank and designated as S24 （588 bp）, S14 （708 bp）, S23 （972 bp）, Szl （971 bp） ,respectively, and 15 S-RNase gene fragments were novel S-RNase genes, temporarily named as S40 （572 bp） （ accession number： HQ342870）, S41 （401 bp） （accession number： HQ342871）, S42 （357 bp） （accession number： HQ342872）, S43 （493 bp） （accession number： HQ342873）, S44 （657 bp） （ accession number： HQ342874）, S45 （1 294 bp） （ accession number： HQ3428570）, S46 （830 bp） （accession number： HQ342876）, S47 （575 bp） （accession number： HQ342877）, S48 （594 bp） （accession number： HQ342878）, S49 （653 bp） （accession number： HQ342879）, S50 （659 bp） （accession number： HQ342880）, S51 （768 bp） （accession number： HQ342881）, S66（1 520 bp） （aceession number： HQ342882）, S65 （594 bp） （accession number： JQ327151）, S66 （709 bp） （accession number： JQ327152）. S-genotypes of the 27 apricot cultivars of Xinjiang were identified including Sz3S14 for Wan- shuhuanna, Hexieke, Beixinnaer, S52S14 for Qiaoerpang, Mantouyulvke, S14S47 for Keziaqia, Yuandanx- ing, Lajiaoxing, S23S48 for Shaeheheiyexing, Kuehetuoyong, Anjianghuanna, S5zS23 for Suogejianali, S52Ssl for Dongxing, S5zS5o for Suluke, S52S4o for Kabakehuanna, S43S41 for Jagedamayishang, Hongdauke, Cui- jianali, Kalahuanna, Kezijianali, S45S42 for Maolaxiao, S45S46 for Tuohuti, S45S44 for Shailaikeyulvke, S49S24 for Luerletuoyong, Huangrouyouxing, S21S65 for Saimaiti, S52S66 for Xiaobaixing. [Conclusion] S-genotypes of the 27 aprieot eultivars in Xinjiang were identified, which provided academic basis for choosing suitable pollination cultivars for eommercial apricot cultivation.