动脉内膜损伤模型中合成型平滑肌细胞的培养

Culture of Synthetic Smooth Muscle Cells Derived from Model of Narrowing Vessel

目的 建立更接近在体情况的合成型血管平滑肌细胞 (VSMC)的培养体系。方法 培养细胞取材于兔动脉内膜损伤后的增生内膜。结果 培养细胞 10天后光镜下表现典型的“峰”、“谷”样排列 ,免疫组化证实是 VSMC,电镜下细胞表现有合成型细胞的特点。结论 直接对合成型 VSMC的培养优于单纯中膜 VSMC培养。

Objective To establish a VSMC cultivating system which is similar close to in vivo condition. [WT5,5”HZ]Methods Cells derived from rabbit hyperplastic intima after arterial intima injury were cultured. Results These cells, identified by transmission electron microscope displayed both ultrastructural features of synthetic SMC and characteristic immunocytochemical staining for smooth muscle myosin. They were arranged in the form of “peak” and “valley”. Conclusion Cultured synthetic SMC is better than SMC cultured from the medium.