人甲状腺乳头状癌细胞对牛-TSH和去甲肾上腺素反应性的实验研究

Research of the Effect of the Cells of Thyroid Papillary Carcinoma to Bovine TSH and Norepinephrine

目的 通过体外实验研究人体甲状腺乳头状癌细胞对牛 -促甲状腺激素 (TSH)、去甲肾上腺素的反应性 ,阐述它们之间的关系 ,观察甲状腺癌细胞生长的影响因素 ,探讨其内分泌治疗的机理。方法 实验标本来自手术病例 ,病理类型为乳头状癌。修理组织、漂洗、剪碎 ,胰酶和胶原酶 型双酶消化 ,96孔板置于 37℃、5 % CO2 、95 %湿度孵箱中培养。用不同浓度的 b TSH和去甲肾上腺素分别刺激 72 h;非放射细胞增殖法测定细胞生长。结果 显示肿瘤细胞对 b TSH无反应 ;而去甲肾上腺素促进癌细胞生长。结论 b TSH不能促进甲状腺乳头状癌细胞生长 ,提示临床上甲状腺癌的 TSH抑制治疗机理有待进一步探讨 ;另外提示甲状腺癌也受到肾上腺素神经内肽的调节。

Objective To investigate the mechanisms of TSH suppressing therapy using T4 on differentiated thyroid cancer. We evaluated the effection of the cells of pappillary carcinoma to bovine TSH and norepinephrine in vitrol. [WT5,5”HZ]Methods The five samples of thyroid papillary carcinomas verified by frozen sections were cut off from the patients with thyroid cancer. They were dispersed by collagenase Ⅰ and trypsin and cultured in bottles, then the carcinoma cells were seeded in 96 well cell culture plates at 37℃ in 5% CO\-2 and 95% humidity for 72 h. The cells in the wells were separately stimulated with different doses of bovine TSH and norepinephrine. [WT5,5”HZ]Results The thyroid cancer cells did not respond to bovine TSH; but were in response to norepinephrine which enhanced the proliferation of thyroid cancer cells. Conclusion The mechanisms of suppression therapy on differentiated thyroid cancer by T4 must be further investigated. Additionally, our results suggest that thyroid cancer is also under the regulation of adrenergic neurotransmitters.