维吾尔族妇女宫颈癌及HPV感染与多种基因表达上调的关系

Relationship of Cervical Cancer of Uighur Women and HPV Infection with the Up-regulation of Multiple Gene Expression

通过对维吾尔族妇女宫颈癌病变的高表达候选基因筛查分析,寻找族群特异的宫颈癌早期预警分子指标及其与HPV感染的相关性。收集维吾尔族妇女患者子宫颈新鲜组织标本85例,其中子宫颈鳞癌53例,慢性宫颈炎32例作为对照。提取组织DNA和RNA,利用HPV特异性PCR引物鉴定HPV感染阳性和型别,采用半定量RT-PCR方法对鉴定7种候选基因的转录表达水平变化。结果表明,宫颈炎或癌旁正常组织中的TK1、SPP1和ARPC2/3 mRNA检出率较低(25%—28%),在宫颈鳞癌中明显增高(62%—66%),其差异显著(P<0.05);但是ANX8、HSPA1A、S100-A9和S100-P基因的转录表达水平变化不明显,其差异也无统计学意义(P>0.05)。从HPV感染的角度分析,HPV阴性患者的TK1和SPP1 mRNA表达检出率较低(50.0%和44.2%);HPV阳性患者很高(82.9%和74.2%),有统计学意义(P<0.05)。研究表明,SPP1、TK1和ARP2/3基因表达上调可能是维吾尔族宫颈癌发生的重要标志,其中TK1和SPP1基因表达水平变化可能对HPV感染有依赖关系。

Previous studies suggest that the profiles of over-expressed genes in cervical carcinoma are very different among populations. The aim of this study is to identify the early warning molecular markers specific to Uighur women by screening the cervical lesions for candidate over-expressed genes. 85 cases of fresh cervical tissues were collected from Uighur women with Cervical Squamous Cell Carcinoma （CSCC） and chronic cervicitis as well as the normal tissue specimens from the surgical excision of uterus. After preparation of the tissue, the total DNA and RNA, HPV positivity and genotypes were screened by the HPV specific primer PCR, and the transcription level of 7 candidate genes was analyzed by the semi-quantitative RT-PCR. The positive rates of the mRNA expressions of the SPP1, TK1 and ARP2/3 genes are found to be relative low in the chronic cervicitis and the normal periphery of the tumor （25%--28%）, and are markedly increased in the CSSC （62%--66%）, with a significant difference （P〈0.05）, but with no significant difference in the expressions of ANX8, HSPA 1A, S100-A9 and S100-P genes. In case of the HPV infection, the rates of TK1 and ARP2/3 gene expressions are very low in the HPV negative cases （50.0% and 44.2%）, relatively high in the HPV positive cases （82.9% and 74.2%）, and with a significant difference （P〈0.05）. The up regulation of SPP1, TK1 and ARP2/3 gene expressions might serve as an important mark of the cervical cancer progression in Uighur women, and the alteration of TK1 and SPP1 gene expressions may be related with the HPV infection.