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碘化油阳离子乳剂在基因给药中的应用

Application of Iodized Oil Cationic Emulsion in Gene Delivery
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摘要 首先制备出粒径为136.5 nm、表面电荷为+24.3mV的碘化油阳离子乳剂,然后以绿色荧光质粒pEG-FP-C1为模型药物,通过在体外细胞培养来观察碘化油阳离子乳剂作为基因载体对机体细胞转染率的影响.体外细胞培养结果显示,碘化油阳离子乳剂复合物的细胞膜穿过率明显提高,转染率远高于裸质粒. Iodized oil cationic emulsion＇ s particle diameter is 136.5nm and surface charge is ＋ 24.3mV. The green-fluorescence plasmid pEGFP-Cl is the model drug, and the genetic carrier which effects transfection efficien- cy of body cell culture in vitro is observed. The experiment results indicate that the cellular membrane permeating percentage rises obviously and its transfection efficiency is higher that that of plasmid.
作者 穆思蒙
机构地区 沈阳药科大学药学院
出处 《洛阳师范学院学报》 2012年第8期46-48,共3页 Journal of Luoyang Normal University
关键词 碘化油 阳离子乳剂 基因给药 绿色荧光 iodized oil Cationic emulsion gene delivery green fluorescence
分类号 Q782 [生物学—分子生物学]
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共引文献16

洛阳师范学院学报
2012年 第8期

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