银杏叶复方对脾虚证小鼠细胞因子、免疫球蛋白和血浆内几种神经肽含量的影响

Effect of Ginkgo biloba compound on cytokines,immunoglobulins,and several plasma neuropeptides in mice with spleen deficiency syndrome

采用利血平复制脾虚证小鼠模型,观察银杏叶复方对实验性脾虚证模型小鼠细胞因子、免疫球蛋白、血浆内几种神经肽含量和体重变化的影响。试验分为三组,即对照组、脾虚证组和银杏叶复方组。结果表明,①脾虚证小鼠IL-1、IL-2含量较对照组显著降低(P<0.05),IL-4、IL-6含量较对照组显著增加(P<0.05),银杏叶复方组上述各项指标与对照组相比差异不显著(P>0.05),但TNF-α含量较对照组和脾虚证组则显著提高(P<0.05)。②脾虚证小鼠的血浆IgG、IgM含量显著低于对照组(P<0.05),银杏叶复方组IgG、IgM含量显著高于脾虚证组(P<0.05),与对照组差异不显著(P>0.05);脾虚证组小鼠血浆IgA含量均低于对照组和银杏叶复方组(P>0.05)。③脾虚证小鼠血浆β-EP(β-内啡肽)含量高于对照组(P>0.05),银杏叶复方组血浆β-EP恢复接近对照组(P>0.05)。④脾虚证小鼠血浆CGRP(降钙基因相关肽)含量较对照组和银杏叶复方组显著提高(P<0.05),NPY(神经肽Y)含量较对照组和银杏叶复方组则显著降低(P<0.05),银杏叶复方组小鼠体重的减轻幅度低于脾虚证组(P>0.05),脾虚证组小鼠体重的减轻幅度显著高于对照组(P<0.05),由于脾虚证小鼠血浆中CGRP过高,NPY含量减少导致摄食行为降低体重下降。结果显示,脾虚证小鼠细胞因子网络调节系统的失衡与其较高的β-EP含量有关,即脾虚证小鼠存在神经-内分泌-免疫网络之间调节失衡,引起脾虚时的机体免疫功能低下,经银杏叶复方治疗后上述免疫失调得到明显纠正。

Spleen deficiency model by reserpine was established in mice to observe the effects of Ginkgo biloba compound on cytokines, immunoglobulins and several plasma neuropeptide. Experimental mice were divided into three groups including control group, spleen-deficiency group and Ginkgo bi/oba compound group. Results showed that, （1） levels of iL-1, IL-2 in spleen-deficiency group were significantly lower than that in control group （P〈0.05）, while IL-4, IL-6 reversed, and no difference was found between compound fed group and control group, but levels of TNF-α in compound fed group was significantly higher than that in control group and spleen deficieney group （P〈0.05）. （2） mice in spleen-deficiency group had lower contents of plasma IgG, IgM （P〈0.05） and IgA （P〉0.05）than those in control group and compound fed group, and no difference was found between compound fed group and control group （P〈0.05）. （3） plasma β-EP level in spleen deficiency group was slightly but not significantly higher than that in control group （P〉0.05）, and β-EP level was compared between compound fed group and control group （P〉0.05）. （4） mice in spleen-deficiency group had higher contents of plasma CGRP （P〈0.05） and lower levels of NPY than those in control and compound fed groups （P〈0.05）, and mice of compound fed group and control group had compared weigh loss （P〉0.05）, while mice in spleen deficiency group presented more severe weight loss than control group （P〈0.05）, which could implicate that extraordinarily high plasma CGRP led to the decline of NPY and consequently caused drop of food-take of spleen deficiency model mice, additionally, perhaps accounted for dysregulation of cytokines nets of nerve-endocdnosis-immune and finally resulted in immune hypofunction which could be fortunately rescued by Gingko biloba compound.