Taguchi法优化Bacillus amyloliquefaciens fmb50产surfactin工业发酵培养基

Optimization using the Taguchi method of the industrial fermentation medium for Bacillus amyloliquefaciens fmb50 for surfactin production

采用田口方法(Taguchi method)对影响surfactin生产的各因素进行筛选,并对显著因子的水平进行优化后得出最佳配比。统计分析结果表明:玉米粉、硝酸铵和PO43-对其产量影响显著。获得高产工业发酵培养基的配方为:玉米粉35g/L,硝酸铵为15g/L,尿素6g/L,PO34-20mmol/L,Mn2+0.5mmol/L,Mg2+0.1mmol/L,Cu2+12.8μmol/L,Fe2+1μmol/L,Ca2+0.5μmol/L。此培养基surfactin产量达2294.28 mg/L,较原有Landy培养基产量提高15%,生产成本降低40%,为实现surfactin的工业化生产提供了基础。

To provide some fundamental data about the industrial scale production of the antimicrobial lipopeptide surfactin, the design of a cheap, high yield and stable industrial medium has been studied. Using the Taguchi method to sereeng the main factors affecting the production of surfactin, the levels of the significant factors were op- timized in order to achieve maximal surfactin yield. The results showed that corn powder, ammonium nitrate and pO34- all had a significant effect on surfactin production. By further optimizing each factor, the optimal media com- position was found to be： corn powder 35 g/L, ammonium nitrate 15 g/L, carbamide 6 g/L, PO34- 20 mmol/L, Mn2＋ 0.5 mmol/L, Mg2＋ 0. 1 mmol/L, Cu2＋ 12.8 μmol/L, Fe2＋ 1 μmol/L, and Ca2＋ 0.5μmol/L. This medium composition afforded 2294.28 mg/L of surfactin, which was 15% higher than the original Landy medium, and the cost was reduced by 40%.