左右利手(爪)小鼠模型抑郁和焦虑样行为研究

A study on depression-and anxiety-like behavior in left-and right-handed mice

目的通过小鼠模型来研究利手是否与抑郁和焦虑样行为有关。方法利用利爪行为测定装置测试了20只C57BL/6J雄性小鼠,共得到12只有明显用爪偏好的小鼠,其中左利爪6只,右利爪6只,对其进行自发活动和强迫游泳以及高架十字迷宫行为测试,观察两组小鼠在各项测试中的表现。结果自发活动测试显示左右利爪小鼠的移动距离和时间差异均无统计学意义;强迫游泳实验中左右利爪小鼠的不动时间的差异无统计学意义;高架十字迷宫实验中,左利爪小鼠进入开放臂的时间明显比右利爪小鼠长【左利爪(47.5±8.689)s vs右利爪(26.53±2.414)s,P<0.05】,差异具有统计学意义。结论提示右利爪小鼠较左利爪小鼠更易出现焦虑样行为,但不同利爪小鼠的抑郁样行为没有差异。

Objective To investigate the relationship between handedness and depression-or anxiety-like be-havior in mice. Methods Behavior measuring ＂handedness＂ devices were used to test 20 C57BL/6J male mice. Af-ter twelve typical paw preference mice, including 6 left-handed and 6 right-handed were identified, these mice un- derwent spontaneous activity, forced swimming and elevated plus-maze behavioral test. Results Neither total moving distance and duration of spontaneous activity nor the immobility time in the forced swimming test were different be- tween left-and right-handed mice. However, Elevated plus-maze test demonstrated that left-handed mice spend more time in the open arm compared with right-handed mice in the [left handed （47.5 ± 8.689）s vs right handed（26.53± 2.414）s, P 〈 0.05]. Conclusion Right-handed mice are more susceptible to anxiety-like behavior but not depres-sion-like behavior than left-handed mice.