拟南芥钙调素结合蛋白参与胁迫响应的研究(英文)

Study on Arabidopsis thaliana Calmodulin-binding Protein Responding to Multiple Stresses

采用实时荧光定量RT-PCR和Northern blotting技术检测了野生型拟南芥中CBP60g基因对丁香假单胞菌和非生物胁迫的响应,并对丁香假单胞菌接种后,野生型拟南芥、cbp60g-1突变体和CBP60g过表达转基因植物中抗逆相关基因的表达变化进行检测。结果显示:(1)在野生型拟南芥中CBP60g基因的表达能被丁香假单胞菌、高盐、冷和机械损伤所诱导。(2)经丁香假单胞菌诱导后病程相关基因PR5和AIG1的表达在过表达转基因植物中明显高于野生型。(3)受干旱和ABA诱导的AtMYB2基因的表达在过表达转基因植物中也高于野生型。研究表明,CBP60g同时参与了拟南芥对生物和非生物胁迫响应。

In this study, real-time PCR or Northern blotting was employed to analyze the expression patterns of the CBP60g gene in wild-type Arabidopsis thaliana after Pseudomonas syringae and abiotie stress treatment. In addition, the expression of pathogen-induced genes in wild-type, cbp6Og-1 mutant and CBP60g overexpression plants after P. syringae inoculation were compared. The results showed. （1）Tran- script of CBP60g was induced by P. syringae,high salt,cold and wounding treatment in wild-type plants. （2）The expression of pathogen-induced genes PR5 and AIG1 in the CBP60g overexpression plants was en- hanced after P. syringae inoculation and was reduced in the mutant. （3）AtMYB2,an abiotic-stress induced gene reported previously,was also induced by Pst DC3000,and reached to a higher level in overexpression plants than in wild-type. Taken together ,these results indicate that CBP60g involved in A. thaliana mufti- ple stresses response.