荷瘤鼠及口腔癌患者热化疗前后T淋巴细胞亚群、白细胞介素-2和肿瘤坏死因子-α水平的变化

Change of T lymphocyte subsets,interleukin-2 and tumor necrosis factor-α in tumor-bearing mice and patients with oral cancer receiving thermo-chemotherapy

目的探讨荷瘤鼠及口腔癌患者热化疗前后T淋巴细胞亚群、白细胞介素(IL)-2和肿瘤坏死因子(TNF)-α活性水平的变化,并初步探讨三者之间的相关性。方法经热化疗处理后,采用甲基噻唑基四唑(MTT)法检测荷瘤鼠淋巴细胞转化指数(LTI)及IL-2、TNF-α水平,MTT法检测口腔癌患者IL-2、TNF-α水平,并采用3H-胸腺嘧啶核苷(3H-TdR)掺入法行淋巴细胞转化实验及CD4+、CD8+T细胞亚群检测。结果高温联合平阳霉素治疗组(HP组)荷瘤鼠LTI、IL-2和TNF-α活性与正常对照组(N组)荷瘤鼠差异无统计学意义(P>0.05),而明显高于平阳霉素治疗组(P组)及不治疗组(NT组)(P<0.01)。临床实验中热化疗后口腔癌患者LTI、CD4+细胞数目及CD4+/CD8+比值明显提高,IL-2和TNF-α活性水平明显升高(P<0.01)。结论荷瘤宿主的LTI及IL-2和TNF-α活性水平在热化疗后明显升高,IL-2和T细胞之间有明显的相关性。

Objective To investigate the change of T lymphocyte subsets,interleukin（IL）-2 and tumor necrosis factor（TNF）-α in the tumor-bearing mice and patients with oral cancer receiving thermo-chemotherapy,and inves-tigate the correlation among them.Methods After treatments,the expression of lymphocyte transformation index（LTI）,IL-2 and TNF-α in the tumor-bearing mice were detected with methyl thiazolyl tetrazolium（MTT）,the expression of IL-2 and TNF-α in the patients with oral cancer were detected with MTT,the expression of LTI,CD4＋ and CD8＋ were detected with 3H-TdR incorporation.Results LTI,IL-2 and TNF-α of thermo-chemotherapy group（HP group） had no significant difference comparing with those of normal mice group（N group）（P0.05）,but which were significantly higher than those of chemotherapy group（P group） and no treatment group（NT group）（P0.01）.In clinical trials,the expression of LTI,CD4＋,CD4＋/CD8＋,IL-2 and TNF-α on oral cancer patients after thermo-chemotherapy were significantly higher than those before thermo-chemotherapy（P0.01）.Conclusion After thermo-chemotherapy, the expression of LTI,IL-2 and TNF-α of tumor-bearing hosts are significantly improved,there is a significant cor-relation between IL-2 and T cell.