摘要
目的建立高效液相色谱法测定金鸡菊中金鸡菊查尔酮含量的方法。方法采用Inertsil ODS-3 C18柱(4.6 mm×250 mm,5μm),以乙腈-1%冰醋酸(80∶20)为流动相,流速1.0 mL/min,检测波长378 nm。结果金鸡菊查尔酮在0.4～4.0μg范围内与峰面积呈良好线性关系,回归方程为Y=31 213X-149 303(r=0.999 9),平均回收率为99.21%,RSD=1.70%(n=5)。结论本方法简便、准确、重复性好,可用于金鸡菊的质量控制。
Objective To establish the HPLC metnod for determination of chalcone in Coreopsis.Methods The Inertsil ODS-3 C18 was used as the stationary phase.The mobile phase consisted of acetonitrile-1% glacial acetic acid（80∶20）.The detecting wavelength was 378 nm and flow rate was 1.0 mL/min.Results The linear range of chalcone was 0.4-4.0 μg（Y=31 213X-149 303,r=0.999 9）.The average recovery was 99.21% and RSD was 1.70%（n=5）.Conclusion The method is simple,convenient and accurate.It can be applied to control the quality of Coreopsis.
出处
《中国中医药信息杂志》
CAS
CSCD
2012年第7期48-49,共2页
Chinese Journal of Information on Traditional Chinese Medicine
关键词
金鸡菊
查尔酮
高效液相色谱法
Objective To establish the HPLC metnod for determination of chalcone in Coreopsis.Methods The Inertsil ODS-3 C18 was used as the stationary phase.The mobile phase consisted of acetonitrile-1% glacial acetic acid（80∶20）.The detecting wavelength was 378 nm and flow rate was 1.0 mL/min.Results The linear range of chalcone was 0.4-4.0 μg（Y=31 213X-149 303,r=0.999 9）.The average recovery was 99.21% and RSD was 1.70%（n=5）.Conclusion The method is simple,convenient and accurate.It can be applied to control the quality of Coreopsis.
