肿瘤转移相关蛋白1在小鼠肝癌诱发过程中的表达与意义

Expression and potential role of metastasis-associated protein 1 in the induced carcinogenesis of mouse liver

目的:建立BALB/c小鼠肝癌动物模型,观察分析肿瘤转移相关蛋白1(MTA1)在建模过程中表达的变化及可能的意义。方法:采用联合二乙基亚硝胺(DEN)/四氯化碳(CCl4)/乙醇的方法诱导正常成年BALB/c雄性小鼠150 d,HE染色观察小鼠肝脏病变情况,免疫组化染色观察MTA1在肝脏病变部位的表达情况。结果:实验组小鼠肝脏镜下依次出现炎症、纤维化和肿瘤性改变。在肝脏病变进展的进程中,MTA1的表达量增加;且肝硬化期,MTA1主要在胞质中表达。结论:MTA1在DEN诱发小鼠肝肿瘤过程中表达量增加、表达位置改变,提示MTA1可能在肝脏肿瘤形成的全程均具有重要作用。

AIM： To establish a hepatocellular carcinoma model of BALB/c mouse and to study the expression and potential role of metastasis-associated protein 1（MTA1） in the carcinogenesis process.METHODS： Normal adult male BALB/c mice were induced by the combined dimethylnitrosamine（DEN）/carbon tetrachloride（CCl4）/alcohol for 150 d.The morphological changes in liver cells and the expression of MTA1 in the liver lesions were observed by HE and immunohistochemical stainings,respectively.RESULTS： The pathological changes of the survivals＇ livers in experimental group were liver inflammation,fibrosis and cancer in sequence.The level of MTA1 increased in the carcinogenesis process,and MTA1 was mainly expressed in the cytoplasm of the cells suffering cirrhosis.CONCLUSION： The changes of the expression sites and quantity of MTA1 in the DEN-induced carcinogenesis of mouse liver indicate that MTA1 may play an important role in the whole process of liver carcinogenesis. [