Bif-1对前列腺癌LNCap细胞生物学行为的影响

Bif-1 gene influences biological behavior of prostate cancer cell line LNCap

目的研究Bif-1基因的过表达对前列腺癌细胞凋亡、增殖以及迁移过程的影响。方法构建Bif-1基因全克隆并转染LNCap细胞,上调细胞中Bif-1基因的表达水平,通过流式细胞术检测细胞转染前后的细胞凋亡情况;通过MTT法检测细胞的增殖能力变化;通过划痕试验检测细胞迁移能力的变化。结果转染Bif-1基因后,Real-time PCR检测发现LNCap细胞中Bif-1基因mRNA水平△△CT高于对照组10倍以上;Western blotting条带光密度值提高2倍以上。实验组细胞凋亡比例升高至31.90%,细胞增殖能力减弱。实验组和对照组中LNCap细胞划痕愈合速度基本相同,差异无统计意义(P>0.05)。结论 Bif-1基因在LNCap细胞中的过表达促进了细胞凋亡,抑制细胞的增殖能力,而对前列腺癌细胞的迁移能力无显著影响。基因有可能作为一个潜在的前列腺癌治疗靶点。

Objective To study the effects of Bif-1 gene over expressed in LNCap cells in apoptosis, proliferation and migration process. Methods Bif-1 gene clone was constructed and transfected into LNCap cells to raise the level of Bif-1 gene expression in LNCap cells. Cell apoptosis was detected by flow cytometry. Cell proliferation was measured by MTr assay and cell migration was detected by scratch test. Results /k/k CT of Bif-1 mRNA level was 10 more than that in the control group detected by Real-time PCR after transfection of the Bif-1 gene into LNCap cells. Bif-1 protein was more than 2 times increased detected by Western blotting. The proportion of apoptosis increased to 31.90% and the proliferation capacity of cells is reduced in experimental groups, but the cell migration capacity with no significant difference between experimental group and control. Conclusion Bif-1 gene overexpression in LNCap cells promoted cell apoptosis, and inhibited cell proliferation, but had no significant effect on prostate cancer cell migration. Bif-1 gene may be a ootential prostate cancer theraneutic target.