黄芩素对星形细胞瘤SHG-44细胞增殖、侵袭迁移的影响

Influence on cell proliferation and invasiveness of human astrocytic glioma cell line SHG-44 by baicalein

目的:观察黄芩素干预人星形细胞瘤SHG-44细胞株后,肿瘤细胞形态、增殖、凋亡、细胞周期以及运动侵袭能力的变化。方法:以体外培养的人星形细胞瘤SHG-44细胞为研究对象,用黄芩素干预后,显微镜观察细胞形态变化,MTT法观察细胞增殖,流式细胞术分析细胞周期,侵袭小室实验观察细胞侵袭力改变。结果:黄芩素对SHG-44细胞增殖的抑制作用随时间的延长和药物浓度的增加,抑制作用逐渐增强,有明显的时间和剂量依赖性;随黄芩素浓度增加,凋亡率增高,差异有统计学意义(P<0.05);黄芩素将肿瘤细胞阻滞于G0/G1期,与对照组比较S期细胞减少近18%,差异有统计学意义(P<0.05);侵袭小室实验表明随着黄芩素干预浓度增加,SHG-44细胞48h后穿过人工基底膜的细胞数量明显更少,与对照组比较差异有统计学意义(P<0.05)。结论:黄芩素能显著抑制SHG-44细胞的增殖、侵袭及迁移,提示黄芩素对星形细胞瘤治疗具有潜在应用价值。

AIM. To investigate the influence on cell proliferation, apoptosis, cell cycle and cell invasiveness after human astrocytic glioma cell line SHG-44 were treated by baicalein with the valious concentrations. METHODS. Human astrocytic glioma cell line SHG-44 were cultured in vitro and treated in various concentrations of baicalein, then MTT method, flow cytometer （FCM） analysis, transwell assay and Western Blot were applied to measure cell growth, cell cycle and cell invasiveness. RESULTS. Bacalein treatment inhibited proliferation of SHG-44 cells in a time and dose-dependent manner. Baicalein treatment induced increasing apoptosis in a dose- dependent manner. Compared with the control, Baicalein caused a significant G0/G1 phase arrest （P〈0.05） and a decrease in the percentage of cells in S-phase by approximately 18% （P 〈 0.05） in a dose-dependent manner. No signifi- cant change for G2/M phase cells was observed. The result of Transwell assay exhibited that cell number through the artificial basal membrane was obviously less when compared with the con- trol after 48 hours （P〈0.05）. CONCLUSION： Bacalein treatment inhibited growth of SHG-44 ceils in a time and dose-dependent manner. Ba icalein can inhibit cell invasion and migration of human astrocytic glioma cell line SHG-44. Baca- lein may be a useful therapeutic strategy for hum astrocytic glioma.