S-腺苷蛋氨酸对肝脏缺血再灌注损伤大鼠炎症因子的影响

Influence of S-ademetionine on inflammatory factor after injury induced by ischemia-reperfusion in rat liver

目的:观察S-腺苷蛋氨酸对SD大鼠肝脏缺血再灌注损伤后HIRI炎症因子的影响。方法:将72只SD大鼠随机分为3组:假手术组(Sham组)、缺血再灌注对照组(IR组)、缺血再灌注腺苷蛋氨酸干预组(SAM组),每组24只。Sham组打开腹腔,游离肝十二指肠韧带,不阻断肝脏血供,20 min后再关腹。IR组和SAM组开腹后游离肝十二指肠韧带,用无创微血管夹夹闭肝蒂,持续20 min后松开血管夹然后关腹。Sham组、IR组术后均予生理盐水1 mL(/kg.d)腹腔注射,SAM组予S-腺苷蛋氨酸100 mg(/kg.d)腹腔注射。3组分别于术后第1、4、7、10天各处死6只大鼠,测定血清和肝组织肿瘤坏死因子(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)含量变化,并取肝左叶部分组织行病理组织学观察。结果:SAM组各个时间点血清和肝组织的TNF-α、IL-1β、IL-6水平均明显低于IR组,差别有统计学意义(P<0.001);SAM组炎症细胞浸润明显少于IR组。结论:S-腺苷蛋氨酸能够降低HIRI时血清与组织炎症因子水平,减少肝组织炎症细胞浸润,保护肝脏组织。

Objective：To investigate the influence of S-ademetionine on inflammatory factor after injury induced by ischemia-reperfusion in rat liver.Methods：The total 72 healthy male Sprague-Dawle rats were randomly divided into three group：Sham operation group（Sham group）,Ischemic-reperfusion group（IR group）,S-ademetionine group（SAM group）,24 rats per group.Rats in sham group were administered with the opening and suture of the abdomen with equal NS by intra-peritoneal injection,rats in ischemic-reperfusion group underwent ischemia-reperfusion with equal NS by intraperitoneal injection,and rats in S-ademetionine group was performed with both10%S-ademetionine and ischemia-reperfusion.All rat ischemia-reperfusion models were established by Pringle’s method.1 hour after 20-minute ischemic-reperfusion,intraperitoneal injection was given with the dosage of 1 mL/（kg·d） NS or 100 mg/（kg·d） S-ademetionine.Blood and liver samples were collected and analyzed for the levels of tumor necrosis factor-α（TNF-α） and interleukin-1β（IL-1β）and interleukin-6（IL-6） and hepatic tissue changes were observed at the 1st,4th,7th,10th day after 20-minute ischemia-reperfusion.Results：The serum and hepatic tissue levels of of TNF-α,IL-1β,IL-6 were significantly lower in SAM group than those in IR group（P〈0.001） at the 1st,4th,7th,10th day after 20-minute ischemia-reperfusion;inflammatory cell infiltration of hepatic tissue were significantly slighter in SAM group than in IR group.Conclusion：S-ademetionine reduce inflammatory factors and inflammatory cells infiltration obviusly and relieve liver injury.