原发性膀胱移行细胞癌中P16基因缺失与突变及临床意义

Homozygous Deletions and Mutations of P16 Gene in Transitional Cell Carcinoma of Bladder and Their Clinical Significance

目的：为探讨Ｐ１６基因的改变与膀胱移行细胞癌生物学行为的关系。方法：采用聚合酶链反应－单链构象多态性分析（ＲＣＲ－ＳＳＣＰ），对４０例膀胱移行细胞癌组织Ｐ１６基因纯合缺失、突变进行了研究。结果：膀胱移行细胞癌中Ｐ１６纯合缺失与突变率在Ⅰ、Ⅱ、Ⅲ级肿癌中分别为７．１％、１８．８％、６０％。在Ｔｉｓ～Ｔ_１期和Ｔ_２～Ｔ_４期分别为５．６％及４０．９％且与肿瘤细胞的分化程度、临床分期明显相关。结论：Ｐ１６基因异常可能在膀胱移行细胞癌的发生发展中起作用。

Objective: To elucidate the relationship between the alteration of P16 gene and the biological behavior of transitional cell carcinoma of bladder. Methods: The deletions and mutations of P16 gene in 40 cases of transitional cell caricinoma of bladder were studied by using PCR - SSCP. Results: The deletion and mutation rate of P16 gene in grade Ⅰ、Ⅱ and Ⅲ tumors were 7.1%, 18.8% and 60% respectively and in stage Tis - T_1 and T_2 - T_4tunors being 5.6% and 40.9%, which was markedly asso- siated with the differentiation and clinical stage of the tumor.Conclusions:The alteration of P16 gene were likely to play an impor- tant role in the tumorigenisis and progression of transitional cell carcinoma of bladder.