摘要
背景与目的:趋化因子受体及其配体在肿瘤特异性转移中发挥重要作用,本研究旨在探讨人趋化因子受体6(human chemokine receptor 6,CCR6)及其配体(CC chemokine ligand 20,CCL20)的表达与结直肠癌肝转移的关系。方法:取液氮保存的50例结直肠癌标本,运用实时荧光定量PCR和免疫组织化学法检测不同组织中CCR6、CCL20 mRNA和蛋白表达情况,分析其差异。结果:CCR6及CCL20 mRNA和蛋白在结直肠癌和肝转移灶中高表达,并且均显著高于癌旁组织。CCR6 mRNA和蛋白在结直肠癌病灶中的表达比在肝转移灶中高。相反,CCL20 mRNA和蛋白在肝转移灶中的表达比在结直肠癌组织中高。结直肠癌组织中CCR6及CCL20 mRNA和蛋白表达与患者的性别、年龄和病理类型无明显关系。结论:CCR6与其配体CCL20特异性结合,可能参与调节介导结直肠癌向肝脏特异性转移过程。
Background and purpose： The human chemokine receptor and its ligand play an important role in tumor metastasis. The study aimed to investigate the expression of human chemokine receptor 6 （CCR6） and its ligand CCL20 in patients with eolorectal liver metastasis, and find out the relationship between the biological performance and the clinical pathological characteristic, which may play an important role in liver metastasis in colorectal cancer. Methods： Fifty patients with colorectal cancer （CRC） taking surgical operation were sampled. CCR6/CCL20 expressions were detected by real-time fluorescent quantitative PCR （real-time QF-PCR） and immunohistochemistry （IHC） in different tissues from CRC patients. Results： CCR6 and CCL20 expressions were significantly higher in primary colorectal cancers and liver metastasis than the surrounding tissue. Both CCR6 mRNA and protein expression were higher in primary coloreetal cancers than in eolorectal cancer liver metastases, while on the contrary, it was higher in colorectal cancer liver metastases for CCL20. There was no evidence showing the connection between CCR6/CCL20 and gender, age or pathological type. Conclusion： Specific molecular interaction between CCR6 and CCL20 may take part in mediating and modulating the colorectal liver metastasis.
出处
《中国癌症杂志》
CAS
CSCD
北大核心
2012年第7期510-516,共7页
China Oncology
基金
国家自然科学基金资助项目(No:30960445)
