胃肠安丸不同提取部位对小鼠肠推进、胃排空作用研究

Pharmacological effect of the different extracts of Weichangan Pill(胃肠安丸) on the intestinal transit and gastric emptying in mice

目的:探讨胃肠安丸及其不同极性部位对小鼠胃排空和小肠推进的影响。方法 :采用酚红排空方法,观察胃肠安丸及其不同极性部位对正常和新斯的明小鼠模型的胃排空和小肠各段推进的影响。结果:胃肠安丸在400mg/kg对胃排空无影响,800mg/kg时能够抑制正常小鼠胃排空,与对照组比较差异显著(77.80±3.45 vs.93.45±0.94%,P<0.01),同时能够促进小肠推进,使得酚红集中在小肠的后段。而对于新斯的明小鼠模型,随着剂量的增加,胃肠安丸与新斯的明共同促进胃排空,但是和模型组比较并无显著性意义,而且其使得小肠中酚红的含量趋于正常。胃肠安丸的石油醚层抑制了胃排空,其它部位促进了胃排空,但是均和空白组对比无显著性差异(P>0.05),并且石油醚层和水层能够促进小肠的推进。不同的极性部位对新斯的明小鼠模型随着极性的增大,胃排空作用亦增加,但是均弱于新斯的明模型组(P>0.05),同时石油醚层和水层表现出抑制小肠的推进作用。结论:胃肠安丸对胃肠动力有一定的调节作用,并且其发挥作用的活性部位为石油醚层。

To investigate the effect of Weichangan Pill and different extracts on the intestinal transit and gastric emptying in mice. Methods： Phenol red was used as a marker to investigate the effects of Weichangan Pill on motility of small intestine and gastric emptying in mice. Re- suits： There was no significant difference between 400 mg/kg groups and the control group, but gastric emptying was significantly inhibited at 800mg/kg group compared with the control group （ gastric emptying： 77.80 -＋ 3.45 vs. 93.45 _＋ 0.94% , P 〈 0.01 ）. Weichangan Pill in- creased the activity of the small intestine, and phenol red was mainly concentrated in the posterior segment. It could not affect the hyper-func- tion of gastric induced by neostigmine. However, it could make the content of phenol red in intestine of neostigmine mice to normal. P.E. extracts of Weiehangan Pill inhibited the gastric emptying in normal mice, and increased the intestinal transit. Other extracts did not affect this function. In neostigmine mice, the four extracts of Weichangan Pill could augment the gastric emptying, but weaker than neostigmine. And neostigmine extract also inhibited the increased intestinal transit. Conclusion： The results suggest that Weiehangan Pill possesses spas- mogenic and spasmolytie properties, and the active parts are neostigmine extracts.