高效液相色谱法测定大鼠生物样品中人参炔醇的方法学考察

The methodology expedition of panaxynol in rat's biological samples by HPLC

目的:建立生物样品中人参炔醇(panaxynol)的高效液相色谱(HPLC)测定方法。方法:通过对精密度、特异性、回收率、稳定性等考察建立了测定方法,得到标准曲线。结果:生物样品中的panaxynol浓度(C)与色谱峰面积(A)均呈现良好的线性关系,回归方程的相关系数r均大于0.99;panaxynol测定方法的精密度高,重现性好,血浆和各组织样品测定的日内精密度和日间精密度RSD都小于10%;回收率较高,满足生物样品回收率在80～120%的范围,相对标准偏差均小于6%;稳定性好,-20℃冷贮放置不影响测定结果。结论:本法的稳定性、精密度、回收率、专属性和定量线性范围均达到了体内药物分析的要求。

To establish methodology of biological samples determination of panaxynol by High Performance Liquid Chromatography （HPLC）. Methods ： The determination methods were estabishded through expedition of accuracy, specificity, recovery, stability, the standard curve was received. Results： The concentration of panaxynol （C） in biological samples and the chromatographic peak area （A） showed a good linear,the correlation coefficient of regression equation （r） were greater than 0.99 ; The determination of panaxynol had high precision, good reproducibility,The day precision and inter-day precision RSD of determination in plasma and various tissue samples were less than 10% ; recovery rate was high to meet the biological samples in the range of 80 to 120% ,relative standard deviations were less than 6% ; sta- bility were good, - 20℃ cold storage did not affect the determination of the results. Conclusion： The stability, precision, recovery, specific and quantitative linear range reached in vivo drug analysis requirements.