食管鳞癌瘤周微脉管生成与淋巴结转移的相关性研究

The relationship between peritumoral micro-angiogenesis and lymph node metastasis in esophageal squamous cell carcinoma

目的:探讨微淋巴管密度、微血管密度与食管鳞癌淋巴结肿瘤转移的关系。方法:选取早期食管鳞癌病理标本,以Podoplanin单克隆抗体标记淋巴管、CD34标记微血管,采用免疫组化SP法检测早期食管鳞癌瘤周微淋巴管密度、微血管密度。对比瘤周微淋巴管密度、微血管密度,确定其与肿瘤淋巴结转移的相关性。结果:食管鳞癌癌周存在微淋巴管及微血管生成,食管鳞状细胞癌淋巴结有转移组和淋巴结无转移组瘤周淋巴管密度及微血管密度有显著性差异。结论:瘤周淋巴管密度与肿瘤淋巴结转移相关,食管鳞癌瘤周淋巴管密度检测可作为判断食管癌淋巴结转移的辅助指标。

Objective ： To investigate the association between micro - angiogenesis and lymph node metastasis in e- sophageal squamous cell carcinoma. Methods： Lymphatic microvessels were identified by immunohistochemical stai- ning using monoclonal antibody against human podoplanin, while micro blood vessels were identified by antibody a- gainst CD34 in 74 cases of esophagus squamous cell carcinoma. Results： Angiogenesis and lymphangiogenesis both happen at peri - tumor region. There were statistical significant differences for both lymphatic microvessel density and micro blood vessels density between lymph node positive and negative groups. Conclusion： The lymphatic microves- sel density can reflect the metastasis of lymph node in esophageal squamous cell carcinoma.