地锦草有效部位对红色毛癣菌羊毛甾醇生物合成的影响

Effect of Euphorbia humifusa extract on lanosterol biosynthesis in dermatophyte

目的:研究地锦草有效部位对红色毛癣菌中羊毛甾醇含量的影响。方法:样品在24孔板中培养七天,经皂化提取、甲醇定容后,采用高效液相色谱法进行定量测定。结果:地锦草有效部位浓度最小为32μg/ml能使真菌中的羊毛甾醇含量发生蓄积(P<0.01),在32μg/ml～128μg/ml之间有明显的剂量依赖性。结论:地锦草有效部位可使真菌中羊毛甾醇蓄积,导致细胞膜麦角甾醇的合成受阻来发挥其抗真菌作用。

To study the effect of Euphorbia humifusa extract on lanosterol biosynthesis in fungus. Methods：The sample was analyzed af- ter saponifing,extracting and methanol voluming. Results：Euphorbia humifusa extract inhibited lanosterol biosynthesis with a dose-dependent manner during 32-128 μg/ml in fungus. Conclusion：Euphorbia humifusa extract has remarkable antifungal activity because of its accumula- tion on lanosterol biosynthesis in fungus.