摘要
结合传统的细菌分离培养法与现代分子生物技术方法 16S rDNA菌群分析法对冰鲜鸭中优势腐败菌进行鉴定。传统分离培养检测到了17株优势腐败菌,16S rDNA菌群分析表明,冰鲜鸭中假单胞菌占绝对优势,达到54%;气单胞菌、肠杆菌是仅次于假单胞菌的第二个类群,都分别占15%;乳酸菌、节杆菌、紫色杆菌以及一株未鉴定的属都分别占4%。
Advantage spoilage bacteria were identified in chilled duck by traditional bacteria isolation method and 16S rDNA flora analysis. 17 advantage spoilage bacteria had been detected in chilled duck by traditional bacteria solation method. 16S rDNA flora analysis showed that dominant spoilage bacteria in chilled ducks was of Pseudomonas, being of 54%. Strains of Aeromonas and Enterobacter were the second groups accounted for 15%. The content of a strans ofLactobacillus, Arthrobacter or Bacillus, as well as an unidentified strains, was accounted for 4%.
出处
《现代食品科技》
EI
CAS
北大核心
2012年第7期728-732,共5页
Modern Food Science and Technology
基金
教育部产学研项目(2009B090300422)
2009年粤港关键领域重点突破项目(2009A020700005)
广州市重大民生攻关项目(2011YZ-00016)
关键词
冰鲜鸭
腐败菌
传统分离培养
16S
rDNA菌群分析法
chilled duck
spoilage bacteria
traditional bacteria isolation methods
16S rDNA flora analysis
