摘要
利用杂交瘤技术制备了诺氟沙星的单克隆抗体,建立了以抗诺氯沙星单克隆抗体为基础的荧光免疫分析方法,工作曲线表明在10～500μg/L浓度范围内呈良好的线性关系,回归方程为:I=31.92 logC-5.0083,相关系数r=0.9965,对诺氟沙星最低检出限达6.09μg/L,抑制中浓度为52.88μg/L,且对其它结构类似喹诺酮类药物均有特异性识别。本法可应用于检测动物性食品中残留的微量诺氟沙星。
A monoclonal antibody （MAb） against norfloxacin was prepared using hybridom a techniques, and indirect competitive Fluorescence immunoassays for norfloxacin （NFLX） was established successfully. The regression equation was I=31.921ogC-5.0083 （r= 0.9965） with the limit of detection about 6.09μg/L. The linear detection was ranged well from 10μg/L to 500μg/L. The cross-reaction tests indicated that most structurally related phenolic compounds did not interfere with the analysis of NFLX. The anti- NFLX antibody had excellent recognition specificity to NFLX. The result developed in this study indicates this method would practicable to detection norfloxacin residues in animal derived food.
出处
《现代食品科技》
EI
CAS
北大核心
2012年第7期856-858,共3页
Modern Food Science and Technology
基金
福建省科技合作计划重点项目(2011Y0035)
福建省科技计划项目(2010Y2004)
关键词
诺氟沙星
单克隆抗体
荧光免疫分析法
残留
norfloxacin
monoclonal antibody
fluorescence immunoassay
residue
