烟草根际解钾菌的筛选与鉴定

Isolation and Identification of the Potassium-releasing Bacteria from Tobacco Rhizosphere

以钾长石粉为唯一钾源的硅酸盐细菌选择性培养基,利用梯度稀释分离法和平板划线分离法对烟草根际土壤的细菌进行筛选。用火焰分光光度法测定培养液内的速效钾含量,以筛选出高效菌株。通过形态观察和16S rDNA序列分析对菌株进行种属鉴定,得到8个高效解钾菌。结果表明,变栖克雷伯氏菌(Klebsiella variicola)、产气肠杆菌(Enterobacter aerogenes)、阴沟肠杆菌(Enterobacter cloacae)、成团泛菌(Pantoea agglomerans)产生速效钾的能力较强。

In this study, we separated and purificated the bacteria for releasing insoluble potassium from soil by a selective medium in which potash feldspar power was treated as the only source of potassium. We diluted the bacteria with gradient and lined on culture plate for separation and purification of the bacteria for release potassium, and used flame spectrophotometer to determine potassium content in nutrient solution, and then selected high efficiency bacteria. The results show that Klebsiella variicola, Enterobacte aerogenes, Enterobacter cloacae and Pantoea agglomerans were good for release available potassium from potash feldspar power. Therefore, these bacteria can be used as candidate strain for oroduction of microbial fertilizer.